| 锚定NDV HN蛋白重组干酪乳杆菌诱导雏鸡黏膜免疫应答 |
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| 引用本文： | 孙景秀,王桂华,侯喜林,余丽芸,田斌,侯瑞峰,朱娉慧,周婷婷,齐浩.锚定NDV HN蛋白重组干酪乳杆菌诱导雏鸡黏膜免疫应答[J].黑龙江八一农垦大学学报,2012(2):43-47. |
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| 作者姓名： | 孙景秀 王桂华 侯喜林 余丽芸 田斌 侯瑞峰 朱娉慧 周婷婷 齐浩 |
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| 作者单位： | 黑龙江八一农垦大学生命科技学院;黑龙江八一农垦大学动物科技学院 |
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| 基金项目： | 黑龙江省科技厅重点攻关课题GB05B501-2 |
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| 摘 要： | 以干酪乳杆菌作为呈递抗原活载体,表达新城疫病毒保护性抗原HN蛋白。重组干酪乳杆菌表达外源蛋白后,经SDS-PAGE、Western blot及间接免疫荧光分析,表明目的蛋白表达并展示到乳酸菌表面。将重组菌及空质粒菌株分别滴鼻、点眼免疫雏鸡,于不同时间检测血清样品中特异性IgG;于三免后不同时间采集雏鸡的泪液、气管洗液、胆汁和肠洗液样品,采用间接ELISA方法检测样品的特异性sIgA;用MTT法检测免疫鸡脾淋巴细胞增殖情况,结果显示重组干酪乳杆菌表达系统能刺激动物黏膜免疫反应和系统免疫反应。
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| 关 键 词： | 新城疫病毒 HN蛋白 干酪乳杆菌 黏膜免疫 |
The mucosal Immune Response Induced in Chicks Immunized with Lactobacillus casei anchoring NDV HN Protein |
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| Sun Jingxiu,Wang Guihua,Hou Xilin,Yu Liyun,Tian Bin,Hou Ruifeng,Zhu Pinghui,Zhou Tingting,Qi Hao.The mucosal Immune Response Induced in Chicks Immunized with Lactobacillus casei anchoring NDV HN Protein[J].Journal of Heilongjiang August First Land Reclamation University,2012(2):43-47. |
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| Authors: | Sun Jingxiu Wang Guihua Hou Xilin Yu Liyun Tian Bin Hou Ruifeng Zhu Pinghui Zhou Tingting Qi Hao |
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| Institution: | 1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319; 2.College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University) |
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| Abstract: | Neweastle disease virus(NDV) HN antigen was expressed by the recombinant Lactobacillus casei.The HN protein was surface-displayed on the L.casei verified by Immunofluorescence Microscopy,SDS-PAGE and Western blot.Intranasal and eye dropping immunized chicks was performed with the recombinant strain L.casei harboring pLA-HN or pLA.Specific anti-HN IgG antibody in the serum and specific anti-HN secret immunoglobulin A(sIgA) antibody in the lung and intestine fluid,tear and bile of chicken were detected by indirect ELISA.T-cell proliferation responses were investigated by MTT test.The results demonstrated that the recombinant L.casei could induce the mucosal and systemic immunity. |
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| Keywords: | neweastle disease virus hemagglutinin-neuraminidase protein Lactobacillus casei mucosal immunization |
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