| 猪脾脏转移因子的制备及其活性的检测 |
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| 引用本文： | 张萍,马春全,李冬红,邱美兰,黄娟娟,杨焕民.猪脾脏转移因子的制备及其活性的检测[J].黑龙江八一农垦大学学报,2007,19(3):68-71. |
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| 作者姓名： | 张萍 马春全 李冬红 邱美兰 黄娟娟 杨焕民 |
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| 作者单位： | 1. 黑龙江八一农垦大学动物科技学院,大庆,163319
2. 佛山科学技术学院 |
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| 基金项目： | 广东省科技攻关计划
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佛山市科技专项基金 |
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| 摘 要： | 采用冻融–透析法提取了猪脾脏转移因子(TF),并对其生物活性进行了检测。结果表明,TF可增强小鼠腹腔巨噬细胞的吞噬功能,增加E玫瑰花环的形成率,表明TF具有免疫增强作用,可为生产和临床应用提供依据。
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| 关 键 词： | 转移因子 巨噬细胞 吞噬率 吞噬指数 E玫瑰花环 |
| 文章编号： | 1002-2090（2007）03-0068-04 |
| 收稿时间： | 2007-03-22 |
| 修稿时间： | 2007-03-22 |
Preparation of Transfer Factor of Porcine Spleen and Detection of its Activity |
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| ZHANG Ping,MA Chun-quan,LI Dong-hong,QIU Mei-lan,HUANG Juan-juan,YANG Huan-min.Preparation of Transfer Factor of Porcine Spleen and Detection of its Activity[J].Journal of Heilongjiang August First Land Reclamation University,2007,19(3):68-71. |
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| Authors: | ZHANG Ping MA Chun-quan LI Dong-hong QIU Mei-lan HUANG Juan-juan YANG Huan-min |
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| Abstract: | Transfer factor was extracted by freeze-thaw-dialysis from porcine spleen. The activity of transfer factor extracted was analyzed by the mouse peritoneal macrophages phagocytize trial and E-rosette test. The results showed that TF could enhance the phagocytosis of mouse peritoneal macrophages and increase the E-rosette formation. It was showed that TF had immune-enhancing effect, it would be able to provide the basis for produce and clinical application. |
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| Keywords: | transfer factor macrophages phagocytic rate phagocytic index E-rosette |
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