| 美洲品系葡萄叶多酚的提取工艺优化及抗氧化活性研究 |
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| 引用本文： | 罗英花,王晓微,臧延青,金成浩.美洲品系葡萄叶多酚的提取工艺优化及抗氧化活性研究[J].黑龙江八一农垦大学学报,2016(4):55-60. |
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| 作者姓名： | 罗英花 王晓微 臧延青 金成浩 |
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| 作者单位： | 1. 黑龙江八一农垦大学动物科学技术学院,大庆,163319;2. 黑龙江八一农垦大学食品学院;3. 黑龙江八一农垦大学生命科学技术学院 |
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| 基金项目： | 黑龙江省留学归国人员科学基金项目(LC2015036);黑龙江省博士后科研启动资助项目(LBH-Q13132);留学人员科技活动项目择优资助;学成、引进人才科研启动计划项目(XYB2013-24);黑龙江省大学生创新创业训练计划项目(201510223003) |
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| 摘 要： | 以美洲品系葡萄叶为原料,研究提取液浓度、温度、时间及料液比对多酚提取率的影响,通过正交实验优化工艺条件,并对体外抗氧化效果进行了研究。结果表明:最佳工艺条件为乙醇浓度50%,浸提温度70℃,浸提时间60 min,料液比1∶15,多酚提取率可达1.72%。美洲品系葡萄叶多酚对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、羟基自由基(·OH)和超氧阴离子自由基(O2-·)具有良好的清除能力,其清除率在样品浓度为1.0 mg·m L-1时,分别为L9:87.74%、64.92%和65.00%;L10:86.15%、58.00%和51.20%。
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| 关 键 词： | 多酚 美洲品系葡萄叶 提取 自由基 抗氧化活性 |
Study on the Extraction Process Optimization of Polyphenols from American Strain of Grape Leaves and its Antioxidant Activity |
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| Luo Yinghua;Wang Xiaowei;Zang Yanqing;Jin Chenghao.Study on the Extraction Process Optimization of Polyphenols from American Strain of Grape Leaves and its Antioxidant Activity[J].Journal of Heilongjiang August First Land Reclamation University,2016(4):55-60. |
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| Authors: | Luo Yinghua;Wang Xiaowei;Zang Yanqing;Jin Chenghao |
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| Institution: | Luo Yinghua;Wang Xiaowei;Zang Yanqing;Jin Chenghao;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;College of Food Science,Heilongjiang Bayi Agricultural University;College of Life Science and Technology,Heilongjiang Bayi Agricultural University; |
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| Abstract: | American grape leaves were used as raw materials to study the effect of extraction solvent,temperature,time,and solid to liquid ratio on the extraction rate of polyphenols,the process conditions were optimized by orthogonal experiment,and antioxidant activities were tested as well.The results showed that the optimized extraction conditions were as follows:extraction solvent 50%ethanol,temperature 70℃,time of 60 min,solid to liquid ratio 1∶15. Under these conditions,the extraction rate of polyphenols was 1.72%. The polyphenols extraction showed strong scavenging activities on 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,hydroxyl redical (·OH)and superoxide redical (O2-·)methods,the scavenging rates for the radicals were 87.74%、64.92% and 65.00%(L9);86.15%、58.00%and 51.20%(L10),respectively,when the concentration was 1.0 mg·mL-1. |
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| Keywords: | polyphenols american strain of grape leaves extraction free redical antioxidant activity |
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