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| 单核细胞增生性李斯特菌mpl基因的克隆及其原核表达载体的构建 | |
| 引用本文： | 王璐璐,侯广玉,杨玉英.单核细胞增生性李斯特菌mpl基因的克隆及其原核表达载体的构建[J].黑龙江八一农垦大学学报,2011,23(1):60-62. |
| 作者姓名： | 王璐璐 侯广玉 杨玉英 |
| 作者单位： | 1. 黑龙江八一农垦大学动物科技学院,大庆,163319;哈尔滨维科生物技术开发公司 2. 黑龙江八一农垦大学动物科技学院,大庆,163319;牡丹江医学院 3. 黑龙江八一农垦大学动物科技学院,大庆,163319 |
| 基金项目： | 黑龙江省研究生创新科研项目 |
| 摘 要： | 研究对单增李斯特菌Mpl蛋白进行了基因克隆及表达载体的构建.通过PCR方法对标准株单增李斯特菌菌株mpl基因进行扩增,并将其克隆至pET32 a(+)上构建表达载体.经DNA序列测定分析,扩增出的基因与GenBank发表的单增李斯特菌mpl基因序列EF183452和EF183453的同源性为99.7%,氨基酸同源性为1... |
| 关 键 词： | 单增李斯特菌 mpl基因 克隆 |
Expression vector of Listeria monocytogenes and mpl gene cloning |
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| Wang Lulu,Hou Guangyu,Yang Yuying.Expression vector of Listeria monocytogenes and mpl gene cloning[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(1):60-62. | |
| Authors: | Wang Lulu Hou Guangyu Yang Yuying |
| Institution: | 1(1.College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319; 2.WeiKe Biotechnology;3.Mudanjiang Medical College) |
| Abstract: | This research was carried out on the Listeria monocytogenes Mpl protein through the gene cloning.Listeria monocytogenes Mpl gene was amplified from type strain by PCR and cloned into expression vector pET32a(+).The nucleotide sequence of Listeria monocytogenes mpl gene was compared and analyzed with accession No.EF183452 and EF183453 published previously in Gene Bank.Sequence analysis showed that the gene shared 99.7% nucleotide homology and 100% Amino Acid homology.The results of the study showed that 1 533 bp length mpl gene of Listeria monocytogenes strain was successfully amplified and cloned,which laid a foundation for a study on the function of Mpl protein. |
| Keywords: | Listeria monocytogenes mpl gene clone |
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