| 亚硫酸钠对大豆胰蛋白酶抑制剂钝化作用研究 |
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| 引用本文： | 秦学功,王海鹏,安红波,吴志军.亚硫酸钠对大豆胰蛋白酶抑制剂钝化作用研究[J].黑龙江八一农垦大学学报,2011,23(1):31-33. |
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| 作者姓名： | 秦学功 王海鹏 安红波 吴志军 |
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| 作者单位： | 黑龙江八一农垦大学生命科学技术学院,大庆,163319 |
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| 基金项目： | 黑龙江农基总局课题项目 |
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| 摘 要： | 探索大豆中胰蛋白酶抑制剂有效、快速的钝化方法.通过正交试验设计研究温度、时间、亚硫酸钠浓度对抑制因子的钝化影响,采用酚红法和国家标准法(GB/T8622-2006)对钝化后大豆中脲酶活性进行定性、定量检测.最优钝化工艺条件为:温度75℃、时间60 min、No2SO3浓度0.03 mol·L-1.亚硫酸钠法是一种简便、...
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| 关 键 词： | 亚硫酸钠 胰蛋白酶抑制剂 钝化 脲酶活性 |
Passivation Effect of Sodium Sulfite on Soybean Trypsin Inhibitor |
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| Qin Xuegong,Wang Haipeng,An Hongbo,Wu Zhijun.Passivation Effect of Sodium Sulfite on Soybean Trypsin Inhibitor[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(1):31-33. |
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| Authors: | Qin Xuegong Wang Haipeng An Hongbo Wu Zhijun |
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| Institution: | (College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319) |
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| Abstract: | Objective: To explore the effective and rapid passivation of the anti-nutritional factors in soybean.Methods: The passivation conditions were optimized with orthogonal test design concerning temperature,time and sodium sulfite concentration.Determination of urease activity in soybean after passivation with red phenol assay and GB/T 8622-2006 assay.Results: The optimal technological parameters:passivate temperature was 75 ℃,passivate time was 60 min and sodium sulfite concentration was 0.03 mol·L-1.Conclusion: The sodium sulfite method is a feasible and effective passivation treatment. |
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| Keywords: | sodium sulfite soybean trypsin inhibitor passivate urease activity |
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