| 糖脂类表面活性剂产生菌株的筛选 |
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| 引用本文： | 李涛,阳辛凤.糖脂类表面活性剂产生菌株的筛选[J].热带农业工程,2009,33(1). |
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| 作者姓名： | 李涛 阳辛凤 |
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| 作者单位： | 海南大学食品学院,海南儋州571737 |
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| 摘 要： | 在海南不同地点共采集15批样品，经富集培养、血平板和蓝色凝胶平板分离，得到200株菌株，经排油圈直径法复筛，其中有6株表面活性较高。以表面活性较高的菌株S211进行摇瓶发酵，对发酵液粗提物进行定性分析、薄层层析（TLC）和红外光谱分析（IR）。结果表明，菌株S211产糖脂类生物表面活性剂，且具有良好的乳化活性。
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| 关 键 词： | 生物表面活性剂 筛选方法 糖脂 |
Screening of Glycolipid Biosurfactant-producing Microorganisms |
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| LI Tao YANG Xinfeng.Screening of Glycolipid Biosurfactant-producing Microorganisms[J].Tropical Agricultural Engineering,2009,33(1). |
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| Authors: | LI Tao YANG Xinfeng |
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| Institution: | LI Tao YANG Xinfeng (College of Food Science and Technology,Hainan University,Danzhou,Hainan 571737) |
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| Abstract: | In Hainan 15 oil-contaminated soil and water samples were collected from different locations and then enrichment cultured, and 200 strains isolated on blood agar and blue agar by hemolytic activity assay. Six strains had higher surface activity in fermentation broth. The strain S211 had a higher surface activity than any other strains. TLC and IR analysis showed that biosurfactant produced by S211 belonged to glycolipids and had good emulsifying activity. |
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| Keywords: | biosurfactant screening method glycolipids |
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