| 鸦胆子组织培养技术研究 |
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| 引用本文： | 李克烈,王荣香,陈伟,马千全,徐立.鸦胆子组织培养技术研究[J].热带农业工程,2011(1):31-33. |
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| 作者姓名： | 李克烈 王荣香 陈伟 马千全 徐立 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所农业部热带作物种质资源利用重点开放实验室; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(No.PZS039) |
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| 摘 要： | 选用鸦胆子健壮幼嫩茎尖作为组培试验材料,进行组培快繁的研究。结果表明:诱导愈伤组织形成的培养基组合为MS+6-BA2mg/L+NAA0.2mg/L,有利于愈伤组织生长;不定芽分化的培养基为MS+6-BA0.5mg/L+NAA0.05mg/L,有利于不定芽的分化;1/2MS+NAA1mg/L有利于组培苗不定根的形成。
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| 关 键 词： | 鸦胆子 愈伤组织 再生植株 |
Tissue Culture Technology of Brucea javanica (L.) Merr |
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| LI Kelie WANG Rongxiang CHEN Wei MA Qianquan XU Li.Tissue Culture Technology of Brucea javanica (L.) Merr[J].Tropical Agricultural Engineering,2011(1):31-33. |
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| Authors: | LI Kelie WANG Rongxiang CHEN Wei MA Qianquan XU Li |
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| Institution: | LI Kelie WANG Rongxiang CHEN Wei MA Qianquan XU Li (Tropical Crops Genetic Resources Institute / Ministry of Agriculture Key Laboratory For Utilization of Tropical Crops Germplasm Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737) |
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| Abstract: | Vigorous and healthy shoot tips of Brucea javanica was selected as explants, and used to carry out the plant tissue culture technology research. The results showed that plant hormone combination of MS + 6-BA 2 mg/L + NAA 0.2 mg/L was good for callus induction, MS + 6-BA 0.5 mg/L +NAA 0.05 mg/L was suitable for adventitious bud formation,1/2MS + NAA 1 mg/L was good for root formation. |
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| Keywords: | Brucea javanica callus regenerated plantlet |
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