沙梨花粉原位萌发与花粉管生长特性

用荧光显微镜对自交不亲和强度不同的梨品种丰水和菊水自花授粉及以丰水为母本的异花授粉后的花粉萌发及花粉管生长特性进行观察。结果表明，自花与异花花粉在柱头上均有较高的萌发率，高达95％以上，且二间没有显的差异。自花与异花花粉管在花柱内的生长速度及停止生长的位置不同，丰水自花花粉管生长到花柱1／3处就完全停止生长，表现为强自交不亲和性，而菊水自花花粉管大部分也在花柱1／3处停止生长，但仍有少量长至花柱基部，表现为弱自交不亲和性；而异花授粉的花粉管虽然在花柱内也有停止生长现象，但大量的花粉管能长到花柱基部，表现为杂交亲和性。花柱内自花授粉的花粉管在停止生长之前有弯曲、先端膨大变形等现象，而异花授粉的花粉管没有此现象。

Characteristics of pollen germination and pollen tube growth in in vivo sand pear styles

Two pear cultivars with different self incompatible strength,Housui and Kikusui,were self pollinated and Housui was pollinated with the pollen of Kikusui,respectively.Following characteristics of pollen germination and pollen tube growth were observed with fluorescence microscope.Both the self and cross pollinated pollen grains on the stigma had a high percentage of pollen germination that was up to over 95%,and there was no distinct difference between them.However,the speed of pollen tube growth and the place where pollen tube growth arrested of both in in vivo style were different.The pollen tube growth of Housui self pollinated completely arrested at the upper one third of the style,which behaved strong self incompatibility.While the arrest of pollen tube growth of Kikusui self pollinated most occurred at the upper one third of the style,only some pollen tubes still grew to the style bottom,which made Kikusui behave as a weakly self incompatible cultivar.Although some cross pollinated pollen tube growth arrested in the styles,lots of pollen tubes still grew to the style bottom,which behaved cross compatibility.The self pollinated pollen tubes in the styles often followed curve,swell and front deformation before arresting growth,but not the cross pollinated ones in the styles.