| 嗜水气单胞菌HEC毒素单克隆抗独特型抗体研究 |
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| 引用本文： | 陈琼,陆承平.嗜水气单胞菌HEC毒素单克隆抗独特型抗体研究[J].南京农业大学学报,1994,17(3):86-90. |
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| 作者姓名： | 陈琼 陆承平 |
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| 作者单位： | 南京农业大学动物医学系，厦门市家畜检疫站 |
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| 摘 要： | 用嗜水气单胞菌ＨＥＣ毒素免疫Ｂａｌｂ／Ｃ小鼠，通过一次性杂交瘤融合，在筛选ＨＥＣ毒素单克隆抗体（ＭｃＡｂ１）的同时，用兔抗ＨＥＣ毒素血清（ＰＡｂ）包板的ＥＬＩＳＡ方法，筛选到两株抗ＨＥＣ毒素的单克隆抗独特型抗体（ＭｃＡｂ２）。两株ＭｃＡｂ２腹水ＥＬＩＳＡ效价分别为１：２０４８０和１：５１２０，属于ＩｇＧ１亚类。ＨＥＣ毒素的模拟抗原ＭｃＡｂ２不能溶解人的Ｏ型红细胞，但能与相应的ＰＡｂ反应，并能阻断ＰＡｂ与ＨＥＣ毒素的结合。将ＭｃＡｂ２连接到同源小鼠红细胞（ＭＲＢＣ）后免疫小鼠，用ＨＥＣ毒素包板的ＥＬＩＳＡ检测其血清，呈阳性结果，说明ＭｃＡｂ２能模拟ＨＥＣ刺激机体产生抗ＨＥＣ毒素的抗体（Ａｂ３）。
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| 关 键 词： | 嗜水气单胞菌 HEC毒素 抗独特型抗体 一步法 |
THE MONOCLONAL ANTI-IDIOTYPIC ANTIBODIES MIMIC HEC TOXIN PRODUCED BY AEROMONAS HYDROPHILA |
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| Chen Qiong,Lu Chengping and Chen Huaiqing.THE MONOCLONAL ANTI-IDIOTYPIC ANTIBODIES MIMIC HEC TOXIN PRODUCED BY AEROMONAS HYDROPHILA[J].Journal of Nanjing Agricultural University,1994,17(3):86-90. |
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| Authors: | Chen Qiong Lu Chengping and Chen Huaiqing |
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| Abstract: | While screening monoclonal antibodies(McAb1)against HEC toxin produced by Aeromonas hydrophila,two hybridomas secreting monoclonal anti-idiotypic antibodies(McAb2) mimic HEC toxin wire selected.The ELISA titre of the two McAb2 were 1:20 480 and 1:5 120 respectively.Hemolytic acitivity of the McAb2 was negative.But they bound rabbit polyclonal antibodies(PAb)against HEC toxin in Dot-ELISA and inhibited the binding of PAb to HEC toxin in a blocked ELISA.One of the McAb2 conjugated syngenic mouse red blood cell(MRBC)might serve as a surrogate antigen and induce specific immune reactivity to the natural antigen. |
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| Keywords: | Aeromonas hydrophila HEC toxin anti-idiotypic antibody one step selection |
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