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3种茎线虫rDNA区的PCR-RFLP分析
引用本文:季镭,王金成,杨秀丽,黄国明,林茂松.3种茎线虫rDNA区的PCR-RFLP分析[J].南京农业大学学报,2006,29(3):39-43.
作者姓名:季镭  王金成  杨秀丽  黄国明  林茂松
作者单位:1. 南京农业大学植物保护学院,江苏,南京,210095
2. 天津出入境检验检疫局,天津,300456
摘    要:采用PCR-RFLP技术对来自国内外的10个茎线虫属群体进行了限制性片段长度多态性分析(RFLP),发现甘薯茎线虫(Ditylenchus destructor)不同群体间存在基因分化现象。来自山东的2个群体Dell和Dely的酶切图谱与来自其他地区的线虫群体相比明显不同。对甘薯茎线虫的近似种鳞球茎茎线虫(D.dipsaci)以及食菌茎线虫(D.myceliophagus)的分析结果显示,通过ITS-RFLP技术建立的酶切图谱可以很好地将3种茎线虫区分开来。

关 键 词:甘薯茎线虫  鳞球茎茎线虫  食菌茎线虫  酶切图谱
文章编号:1000-2030(2006)03-0039-05
收稿时间:12 27 2005 12:00AM
修稿时间:2005年12月27

PCR-RFLP patterns for differentiation of three Ditylenchus species
JI Lei,WANG Jin-cheng,YANG Xiu-li,HUANG Guo-ming,LIN Mao-song.PCR-RFLP patterns for differentiation of three Ditylenchus species[J].Journal of Nanjing Agricultural University,2006,29(3):39-43.
Authors:JI Lei  WANG Jin-cheng  YANG Xiu-li  HUANG Guo-ming  LIN Mao-song
Institution:1. College of Plant Protection, Nanjing Agricultural Uiniversity, Nanjing 210095, China; 2. Tianjin Entry-Exit Inspection and Qurantine Bureau, Tianjin 300456, China
Abstract:The internal transcribed spacer regions of 10 populations of Ditylenchus were analyzed using PCR-RFLP techniques. The results showed that two ITS-RFLP patterns were found in Ditylenchus destructor and 2 populations of D. destructor from Shandong Province, Dell and Dely, had unique enzyme-digestion pattern compared to other populations of this species. The study also sup- ported that PCR-ITS-RFLP method was a valuable tool for differentiation of D. destructor, D. dipsaci and D. myceliophagus.
Keywords:ITS-RFLP
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