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| 白介素2基因与猪圆环病毒2型ORF_2基因真核双表达质粒的构建 | |
| 引用本文： | 潘群兴,何孔旺,温立斌,郭容利,黄克和.白介素2基因与猪圆环病毒2型ORF_2基因真核双表达质粒的构建[J].南京农业大学学报,2009,32(1). |
| 作者姓名： | 潘群兴 何孔旺 温立斌 郭容利 黄克和 |
| 作者单位： | 1. 江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心,江苏,南京,210014;南京农业大学动物医学院,江苏,南京,210095 2. 江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心,江苏,南京,210014 3. 南京农业大学动物医学院,江苏,南京,210095 |
| 基金项目： | 国家重点基础研究发展规划(973计划) |
| 摘 要： | 应用基因重组技术,构建猪圆环病毒2型ORF2基因与白介素2基因(IL-2) 的真核双表达载体并将其转染CHO细胞,通过RT-PCR、间接ELISA、Western-blot、间接免疫荧光检测(IFA) 等方法验证基因的表达情况.结果表明:成功构建了猪圆环病毒2型ORF2基因和IL-2基凶的真核双表达质粒pIRES-ORF2-IL-2,该质粒可在体外同时表达ORF2和IL-2. |
| 关 键 词： | 猪圆环病毒2型 ORF2基因 IL-2基因 DNA疫苗 |
Construction of eukaryotic co-expression plasmid harboring ORF_2 gene of porcine circovirus type 2 and IL-2 gene |
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| PAN Qun-xing,HE Kong-wang,WEN Li-bin,GUO Rong-li,HUANG Ke-he.Construction of eukaryotic co-expression plasmid harboring ORF_2 gene of porcine circovirus type 2 and IL-2 gene[J].Journal of Nanjing Agricultural University,2009,32(1). | |
| Authors: | PAN Qun-xing HE Kong-wang WEN Li-bin GUO Rong-li HUANG Ke-he |
| Institution: | 1.Institue of Veterinary Medicine/Key Laboratory of Animal Diseases Diagnosis and Immunology;Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China;2.College of Veterinary Medicine;Nanjing Agricultural University;Nanjing 210095;China |
| Abstract: | The eukaryotic co-expression plasmid pIRESORF2-IL-2 was constructed by inserting porcine circovirus type 2(PCV2)ORF2 gene and IL-2 into pIRES vector using recombinant DNA technology and transfected into CHO cells.Expression of ORF2 gene of PCV2 and IL-2 in vitro was detected by RT-PCR,iELISA,Western-blot and IFA.The results indicated that co-expression plasmid harboring genes of ORF2 of PCV2 and IL-2 was successfully constructed and the plasmid could simultaneously express ORF2 gene of PCV2 and IL-2 in CHO ... |
| Keywords: | porcine circovirus type 2 ORF2 gene IL-2 gene DNA vaccine |
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