| 彩色马蹄莲细菌性软腐病菌的鉴定及其群体感应淬灭的研究 |
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| 引用本文： | 谷春艳,范加勤,杨雪,胡白石,刘凤权,张永春.彩色马蹄莲细菌性软腐病菌的鉴定及其群体感应淬灭的研究[J].南京农业大学学报,2009,32(3). |
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| 作者姓名： | 谷春艳 范加勤 杨雪 胡白石 刘凤权 张永春 |
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| 作者单位： | 1. 南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室,江苏,南京,210095
2. 上海市农业科学院花卉研究中心,上海,201106 |
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| 基金项目： | 江苏省农业三项工程项目(SX，江苏省科技兴农项目，上海市农业科技攻关项目(沪农科攻字 |
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| 摘 要： | 近几年南京种植的彩色马蹄莲软腐病发生严重,从不同品种的彩色马蹄莲不同部位病组织中分离到6个菌株,经形态特征与培养性状比较、生理生化测试、16S rDNA序列分析和致病性测定,鉴定为胡萝卜软腐果胶杆菌胡萝卜亚种(Pectobacterium carotovora subsp.carotovora,P.c.c).信号分子检测结果表明,分离菌株可产生并释放出N-酰基-高丝氨酸内酯(AHLs)类信号分子.利用PCR技术扩增土壤根癌农杆菌中的attM基因,其编码的AttM解酯酶可以显著降解AHLs,在离体条件下可有效地减弱该病原菌的致病性,这为有效控制彩色马蹄莲细菌性软腐病提供了一种新途径.
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| 关 键 词： | 彩色马蹄莲 细菌性软腐病 病原菌鉴定 群体感应淬灭 |
Identification of the pathogen and quorum quenching study on bacterial soft rot of colored calla lily |
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| GU Chun-yan,FAN Jia-qin,YANG Xue,HU Bai-shi,LIU Feng-quan,ZHANG Yong-chun.Identification of the pathogen and quorum quenching study on bacterial soft rot of colored calla lily[J].Journal of Nanjing Agricultural University,2009,32(3). |
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| Authors: | GU Chun-yan FAN Jia-qin YANG Xue HU Bai-shi LIU Feng-quan ZHANG Yong-chun |
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| Institution: | 1.College of Plant Protection/Key Laboratory of Monitoring and Management of Plant Diseasesand Insects;Ministry of Agriculture;Nanjing Agricultural University;Nanjing 210095;China;2.Flowers Research Center;Shanghai Academy of Agricultural Sciences;Shanghai 201106;China |
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| Abstract: | Bacterial soft rot occurred seriously in colored calla lily(Zantedeschia spp.)in Nanjing,China.Six isolates from different parts of rotted calla lily were identified as Pectobacterium carotovora subsp.carotovora by morphological observation,biochemical and physiological characteristics,16S rDNA sequences analysis and pathogenicity determination.The results showed that the isolated strains produced N-acyl homosrine lactones(AHLs)detected by AHL-bioassay strain JZA1.Furthermore,the lactonase AttM coded by gen... |
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| Keywords: | attM |
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