| 四倍体苇状羊茅和多花黑麦草F_1杂种的幼胚培养及再生植株的形态特征 |
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| 引用本文： | 陈文品,曹明树,张航宁,吴琴生,刘大钧.四倍体苇状羊茅和多花黑麦草F_1杂种的幼胚培养及再生植株的形态特征[J].南京农业大学学报,1997(1). |
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| 作者姓名： | 陈文品 曹明树 张航宁 吴琴生 刘大钧 |
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| 作者单位： | 南京农业大学细胞遗传研究所 |
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| 摘 要： | 研究了在直接成苗培养基上不萌动的四倍体苇状羊茅和多花黑麦草杂种幼胚通过诱导愈伤组织获得再生杂种苗的方法。结果表明，杂种愈伤组织随着继代培养时间的延长，分化成苗能力逐步下降，在不改变培养基的情况下，分化能力可保持８个月；改变脱分化培养基中ＮＨ４ＮＯ３或ＫＮＯ３的浓度可显著提高愈伤组织的分化能力。此外，还讨论了再生植株的形态变异
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| 关 键 词： | 四倍体苇状羊茅 多花黑麦草 胚培养 再生苗 形态特征 |
CULTURE OF IMMATURE EMBRYO AND MORPHOLOGICAL CHARACTERS OF ITS REGENERATION PLANT FROM F 1 HYBRID OF TETRAPLOID TALL FESCUE AND ITALIAN RYEGRASS |
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| Chen Wenpin,Cao Mingshu,Zhang Hangning,Wu Qinsheng and Liu Dajun.CULTURE OF IMMATURE EMBRYO AND MORPHOLOGICAL CHARACTERS OF ITS REGENERATION PLANT FROM F 1 HYBRID OF TETRAPLOID TALL FESCUE AND ITALIAN RYEGRASS[J].Journal of Nanjing Agricultural University,1997(1). |
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| Authors: | Chen Wenpin Cao Mingshu Zhang Hangning Wu Qinsheng and Liu Dajun |
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| Abstract: | Regeneration plants were obtained from a hybrid immature embryo of tetraploid tall fescue ( Festuca arundinacea var. glaucascens Boiss.) and Italian ryegrass ( Lolium multiflorum Lam.) through callus induction and regeneration, which failed to germinate directly on MS medium supplemented with 0.1 mg/L kinetin. The results showed that the differentiation ability of embryo callus decreased with the prolonging of subculture and could be maintained for 8 months; changing the concentration of NH 4NO 3 or KNO 3 in dedifferentiation medium led to significant increase in the callus differentiation ability. The morphological variation of regeneration plants was also described and discussed. |
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| Keywords: | tetraploid tall fescue Italian ryegrass embryo culture regeneration plants morphological characters |
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