不结球白菜胞质雄性不育新种质花蕾和叶片中活性氧代谢的变化

对新育成的不结球白菜雄性不育系及其保持系不同发育阶段的花蕾和营养生长期及盛花期叶片中的活性氧（O2^.-）水平、丙二醛（MDA）含量及抗氧化酶活性进行了比较。结果表明：与保持系花蕾相比，雄性不育系花蕾中O2^.-产生速率、H2O2及MDA含量较高，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性也较高，但抗坏血酸过氧化物酶（APX）活性变化与上述3种酶不同。叶片中虽有保护酶活性的差异，但O2^.-产生速率、H2O2和MDA含量差异不显著。说明不育株与可育株的活性氧代谢的差异主要存在于生殖器官中。

Changes in active oxygen metabolism in the floral buds and leaves of the new CMS line of non-heading Chinese cabbage

The cytoplasmic male sterile(CMS)line and its maintainer line of the newly-bred non-heading Chinese cabbage were taken as experimental materials.The production rate of superoxide anion radical(),contents of malondialdehyde(MDA) and hydrogen peroxide(H_2O_2),and the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT) and ascorbate peroxidase(APX) were determined in buds of different developmental stages and leaves during vegetative and flowering stages.Results showed that there were higher levels of MDA and H_2O_2 contents,higher production rate and higher activities of SOD,POD and CAT in bud of the CMS lines than those in maintainer lines,but activities of APX were different with the three enzymes above.There were differences in activities of protective enzymes in leaves but no significant differences in MDA contents and production rate,which showed that the differences of the active oxygen metabolism between CMS and its maintainer were mainly apparent in reproductive organs.