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烟草赤星病菌糖蛋白激发子诱导烟草抗病防卫反应
引用本文:商明清,梁元存,刘爱新,胡雪光,董汉松.烟草赤星病菌糖蛋白激发子诱导烟草抗病防卫反应[J].南京农业大学学报,2003,26(1):20-23.
作者姓名:商明清  梁元存  刘爱新  胡雪光  董汉松
作者单位:1. 山东省植物保护总站,山东,济南,250100
2. 山东农业大学植物病理学系,山东,泰安,271018
3. 南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095
基金项目:国家自然科学基金项目 ( 3 0 0 80 0 11)
摘    要:用来自赤星病菌弱毒株的糖蛋白激发子处理烟草,能明显抑制烟煤草体内过氧化氢酶(CAT)的活性,增加组织中H2O2的含量,诱导后1dCAT活性就已开始下降,至第6天下降到最低,以后又逐渐恢复,而H2O2含量的变化趋势与CAT活性的变化相反,随CAT活性的降低,组织中H2O2含量逐渐增加,在诱导后第10天达到最大值,激发子处理烟草还可以诱导碱性PR蛋白基因PR1b的表达;同时,烟草对赤星病强毒株的抗性逐渐增强,以诱导后第10天接种抗性最强,且这种抗性是系统性的,水杨酸(SA)处理后CAT及H2O2的变化与上述趋势类似。

关 键 词:烟草  赤星病菌  糖蛋白  激发子  抗病防卫反应  诱导抗性
文章编号:1000-2030(2003)01-0020-04
修稿时间:2002年6月17日

A glycoprotein elicitor from a hypovirulent strain of Alternaria alternata activates pathogen defense pathway in tobacco
Abstract:Tobacco plants treated with a glycoprotein elicitor from the hypovirulent strain TBA 16 of Alternaria alternata, the pathogen that causes brown spot in tobacco,displayed a decreased catalase (CAT) activity and an increased hydrogen peroxide (H 2O 2) content,similarly as did plants treated with salicylic acid(a positive control), compared with the negative control (treatment with sterilized water).CAT activity began to decline one day and got to the lowest level at the sixth day,while H 2O 2 content elevated continuously and reached to the highest level ten days after induction.Application of the elicitor to tobacco induced expression of PR 1b ,a gene encoding basic pathogenesis related protein 1b.Treatment of tobacco with the elicitor induced systemic resistance to the virulent strain TBA28 of the pathogen.
Keywords:elicitor  hydrogen peroxide  defence response  tobacco brown spot  induced resistance
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