| 几种昆虫病原线虫对大蜡螟幼虫血淋巴及其能源物质含量的影响 |
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| 引用本文： | 丁晓帆,林茂松,刘亮山.几种昆虫病原线虫对大蜡螟幼虫血淋巴及其能源物质含量的影响[J].南京农业大学学报,2005,28(3):43-47. |
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| 作者姓名： | 丁晓帆 林茂松 刘亮山 |
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| 作者单位： | 南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095 |
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| 摘 要： | 用异小杆线虫（Heterorhabditis spp．）和斯氏线虫（Steinernema spp．）处理大蜡螟5龄幼虫。结果显示：处理后12h昆虫血细胞开始变形、崩解，且血细胞数量急剧下降，到昆虫接近死亡时（24～32h左右），血细胞数比正常水平下降85％；处理后24h血淋巴总量下降73．61％，酯酶活性不断增强，蛋白含量明显高于对照。总糖含量降低14％～70％。上述结果表明：昆虫病原线虫的侵入破坏了寄主的防御系统，改变了寄主血淋巴能源物质含量。
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| 关 键 词： | 昆虫病原线虫 大蜡螟 血淋巴 酯酶活性 蛋白质 总糖 |
| 文章编号： | 1000-2030(2005)03-0043-05 |
| 收稿时间： | 11 23 2004 12:00AM |
| 修稿时间： | 2004年11月23 |
Effects of entomopathogenic nematodes on energetic contents in hemolymph of Galleria mellonella |
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| DING Xiao-fan,LIN Mao-Song,LIU Liang-shan.Effects of entomopathogenic nematodes on energetic contents in hemolymph of Galleria mellonella[J].Journal of Nanjing Agricultural University,2005,28(3):43-47. |
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| Authors: | DING Xiao-fan LIN Mao-Song LIU Liang-shan |
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| Abstract: | Effects of 5 species of entomopathogenic nematodes(Heterorhabditis spp.and Steinernema spp.) on the energetic contents in hemolymph of 5th larvae of Galleria mellonella was presented.In all the species the number of hemocytes decreased by over 85% and hemocyte shape was obviously changed 24 hours after treatment.The total volume of hemolymph of 5th larvae infected with nematodes decreased by 73.61%,while esterase activity and content of protein increased.The decrease rate of total carbohydrate content was 14p%.The results revealed that the entomopathogenic nematodes destroyed the host immune system and dramatically changed the content of energy source in the hemolymph of the host. |
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| Keywords: | entomopathogenic nematodes Galleria mellonella hemolymph esterase activity protein total carbohydrate |
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