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大肠杆菌DNA作佐剂的鸡新城疫灭活疫苗的免疫效果
引用本文:唐泰山,郭爱珍,陆承平.大肠杆菌DNA作佐剂的鸡新城疫灭活疫苗的免疫效果[J].南京农业大学学报,2003,26(2):76-78.
作者姓名:唐泰山  郭爱珍  陆承平
作者单位:南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095
基金项目:江苏省兽医生物技术中心开放课题
摘    要:用大肠杆菌DNA作为鸡新城疫灭活苗的佐剂接种 15日龄雏鸡 ,以鸡新城疫油乳剂灭活苗为对照 ,用血凝抑制实验检测病毒特异性血凝抑制抗体。结果表明 ,大肠杆菌DNA及油乳剂两种佐剂均能增强特异性血凝抑制抗体的效价 ,二者组间抗体滴度差异不显著 ,但大肠杆菌DNA佐剂组雏鸡的体重明显高于油乳佐剂组 (P <0 0 1)。

关 键 词:大肠杆菌DNA    新城疫  灭活疫苗  佐剂  免疫效果  抗体效价
文章编号:1000-2030(2003)02-0076-03
修稿时间:2002年11月14

Immune enhancement of E. coli DNA on inactivated Newcastle disease vaccine in chickens
TANG Tai shan,GUO Ai zhen,LU Cheng ping.Immune enhancement of E. coli DNA on inactivated Newcastle disease vaccine in chickens[J].Journal of Nanjing Agricultural University,2003,26(2):76-78.
Authors:TANG Tai shan  GUO Ai zhen  LU Cheng ping
Institution:TANG Tai shan,GUO Ai zhen,LU Cheng ping *
Abstract:E coli DNA and oil adjuvant were separately injected into chickens of 15 day old,together with inactivated Newcastle disease virus(NDV).Hemaggtulination inhibition test was performed for detecting specific antibody to NDV in sera of inoculated chickens.The results showed that all fowls developed specific antibody,and there were not significant difference of the antibody titres between E coli DNA group and the oil adjuvant group by statistical analysis ( P <0 05).But the weight of chickens inoculated with E coli DNA was higher than that of chickens inoculated with oil adjuvant ( P <0 01).
Keywords:E  coli  DNA  Newcastle disease virus  chickens  immune enhancement
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