| 芥蓝高频离体再生体系的建立 |
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| 引用本文： | 唐君,张蜀宁,侯喜林,王淑敏,孙成振.芥蓝高频离体再生体系的建立[J].南京农业大学学报,2011,34(2). |
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| 作者姓名： | 唐君 张蜀宁 侯喜林 王淑敏 孙成振 |
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| 作者单位： | 南京农业大学园艺学院/农业部南方蔬菜遗传改良重点开放实验室,江苏南京,210095 |
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| 摘 要： | 以'尖叶芥蓝'、'中花粗心芥蓝'和'迟花芥蓝'3个品种为材料,研究苯基噻二唑脲(TDZ)预处理、不同激素配比、基因型和外植体类型对不定芽再生的影响,建立芥蓝离体高频再生体系.结果表明:3个品种中,'中花粗心芥蓝'再生能力最强;不同外植体类型中再生率由大到小依次为:下胚轴、带柄子叶、子叶块;不定芽再生的最佳激素组合为0.2 mg·L-1NAA+6 mg·L-16-BA;以此激素组合为基础,下胚轴经0.5 mg·L-1TDZ浸泡30 min预处理接种在分化培养基(MS+0.2 mg·L-1NAA+6 mg·L-16-BA+8 g·L-1琼脂+30 g·L-1蔗糖)上不定芽再生率最高,达98.5%.
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| 关 键 词： | 芥蓝 离体培养 高频再生 苯基噻二唑脲(TDZ) |
Establishment of high-frequency in vitro regeneration system of Chinese kale(Brassica alboglabra Bailey) |
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| TANG Jun,ZHANG Shu-ning,HOU Xi-lin,WANG Shu-min,SUN Cheng-zhen.Establishment of high-frequency in vitro regeneration system of Chinese kale(Brassica alboglabra Bailey)[J].Journal of Nanjing Agricultural University,2011,34(2). |
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| Authors: | TANG Jun ZHANG Shu-ning HOU Xi-lin WANG Shu-min SUN Cheng-zhen |
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| Institution: | TANG Jun,ZHANG Shu-ning,HOU Xi-lin,WANG Shu-min,SUN Cheng-zhen(College of Horticulture/Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Based on three Chinese kale(Brassica alboglabra Bailey)varieties of'Sharp Leaf Chinses Kale','Middle Flower Chinese Kale'and'Late-Flower Chinese Kale',we established a high frequency in vitro regeneration system through examining genotype,explant type,different hormones combinations and thidiazuron(TDZ)pretreatment on shoot regeneration effect.The results showed that the'Middle Flower Chinese Kale'held the most powerful regenerative capacity.The descending orders of regeneration rates from different types o... |
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| Keywords: | Chinese kale in vitro culture high-frequency regeneration TDZ |
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