| 我国部分地区大豆疫霉群体遗传分析 |
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| 引用本文： | 唐庆华,崔林开,苗苗,李德龙,阴伟晓,郑小波,王源超.我国部分地区大豆疫霉群体遗传分析[J].南京农业大学学报,2011,34(2). |
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| 作者姓名： | 唐庆华 崔林开 苗苗 李德龙 阴伟晓 郑小波 王源超 |
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| 作者单位： | 1. 南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,江苏南京210095;中国热带农业科学院椰子研究所,海南文昌571339
2. 南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,江苏南京,210095 |
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| 基金项目： | 国家公益性行业(农业)科研专项经费，国家自然科学基金项目，大豆产业技术体系资助项目 |
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| 摘 要： | 为探究我国大豆疫霉菌的遗传多样性,使用13对简单重复序列标记(simple sequence repeats,SSR)引物对来自部分地区的79个大豆疫霉菌分离物进行了DNA指纹分析.13对SSR引物共扩增出33条条带,其中多态性条带比例为97.0%.SSR指纹聚类分析表明:当以相异距离0.79为阈值时,可将79个大豆疫霉菌分离物划分为13个遗传聚类组.多数大豆疫霉菌分离物之间遗传相似性较低,表明我国大豆疫霉在DNA水平上发生了显著的遗传变异,从而具有较丰富的遗传多样性.大豆疫霉菌DNA多态性特征与分离物地理来源之间存在一定相关性,说明不同地区的大豆疫霉菌群体与大豆栽培品种之间的互作很可能是引起大豆疫霉菌发生广泛遗传变异的主要原因.
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| 关 键 词： | 大豆疫霉 群体遗传结构 地理分布 |
Genetic structure analysis of Phytophthora sojae population from several provinces in China |
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| TANG Qing-hua,CUI Lin-kai,MIAO Miao,LI De-long,YIN Wei-xiao,ZHENG Xiao-bo,WANG Yuan-chao.Genetic structure analysis of Phytophthora sojae population from several provinces in China[J].Journal of Nanjing Agricultural University,2011,34(2). |
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| Authors: | TANG Qing-hua CUI Lin-kai MIAO Miao LI De-long YIN Wei-xiao ZHENG Xiao-bo WANG Yuan-chao |
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| Institution: | TANG Qing-hua1,2,CUI Lin-kai1,MIAO Miao1,LI De-long1,YIN Wei-xiao1,ZHENG Xiao-bo1,WANG Yuan-chao1(1.College of Plant Protection/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China,2.Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang 571339,China) |
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| Abstract: | The primary objective of this study was to explore genetic diversity among Chinese Phytophthora sojae isolates.The fingerprints of 79 P.sojae isolates from part of regions of China were determined using 13 simple sequence repeats(SSR)primers.A total of 33 reproducible SSR fragments were scored among 13 primers,of which 97.0% were polymorphic among the Chinese P.sojae population.The cluster analysis with the unweighted pair group method revealed that the 79 isolates of P.sojae were separated clearly into thi... |
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| Keywords: | Phytophthora sojae population genetic structure geographic distribution |
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