| 转CMV—CP基因番茄后代的田间抗病性 |
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| 引用本文： | 汪智渊,薛宝娣.转CMV—CP基因番茄后代的田间抗病性[J].南京农业大学学报,1997,20(1):39-42. |
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| 作者姓名： | 汪智渊 薛宝娣 |
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| 作者单位： | [1]江苏丘陵地区镇江农业科学研究所 [2]南京农业大学 |
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| 摘 要： | 以土壤农杆菌为介导，用叶盘法转化获得的转ＣＭＶ－ＣＰ基因（黄瓜花叶病毒外壳蛋白基因）番茄工程植株，通过连续选择和自交，得到Ｒ１、Ｒ２、Ｒ３、Ｒ４和Ｒ５代的转化番茄植株。１９９３－１９９４年采用人工接种和田间自然发病两种方式鉴定转化植株对ＣＭＶ病毒的田间抗性。结果表明，转基因番茄植株对ＣＭＶ侵染有高度抗性。人工接种Ｒ１、Ｒ２、Ｒ３、Ｒ４代转化植株的防病效果达５０－７３％。有５０％以上的转基因植株始终
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| 关 键 词： | 基因转移 番茄 黄瓜花叶病毒 抗病性 |
RESISTANCE OF CMV CP TRANSGENIC TOMATO TO CMV IN SELFED PROGENIES |
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| Wang Zhiyuan ,Wu Hanzhang ,Xue Baodi and Chen Yongxuan.RESISTANCE OF CMV CP TRANSGENIC TOMATO TO CMV IN SELFED PROGENIES[J].Journal of Nanjing Agricultural University,1997,20(1):39-42. |
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| Authors: | Wang Zhiyuan Wu Hanzhang Xue Baodi and Chen Yongxuan |
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| Institution: | Wang Zhiyuan 1,Wu Hanzhang 1,Xue Baodi 2 and Chen Yongxuan 2 |
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| Abstract: | The transgenic tomato was obtained by transforming Agrobacterium tumefacience with GUS and CMV CP genes residing on the Ti plasmid constructs. The methods of artifical inoculation and natural infection in field were used to test the resistance of CMV CP transgenic tomato plants to CMV in its selfed generations. The result showed that tomato plants transformed with CP genes of CMV had high levels of resistance to CMV and the yield increased significantly in the selfed generations of R 1 to R 5. |
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| Keywords: | CMV CP genes transgenosis tomato cucumber mosaic rirus disease resistance |
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