| 南京大豆根腐病病原物的分离及毒性鉴定 |
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| 引用本文： | 武晓玲,赵晋铭,王永林,孙石,郭娜,唐庆华,王源超,盖钧镒,邢邯.南京大豆根腐病病原物的分离及毒性鉴定[J].南京农业大学学报,2009,32(2). |
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| 作者姓名： | 武晓玲 赵晋铭 王永林 孙石 郭娜 唐庆华 王源超 盖钧镒 邢邯 |
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| 作者单位： | 1. 南京农业大学国家大豆改良中心/作物遗传与种质创新国家重点实验室/农学院
2. 南京农业大学农业部病虫监测与治理重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 公益性行业(农业)科研专项项目，长江学者和创新团队发展计划创新团队项目，江苏省科学技术厅高技术项目 |
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| 摘 要： | 对2005、2006年夏在南京农业大学江浦农场试验田发生的大豆根腐病,采用特异性PCR检测到发病组织中有大豆疫霉,经室内诱捕和分离,从发病田块的土壤和发病植株上共分离到4个大豆疫霉菌株PNJ1、PNJ2、PNJ3和PNJ4.用含有不同抗病基因的14个鉴别寄主测定这4个大豆疫霉菌株的毒力公式,PNJ1和PNJ2为1d,2,3b,3c,4,6,7;PNJ3为1a,1b,1c,1d,1k,2,3b,3c,5,7;PNJ4为1a,1b,1c,1d,1k,2,3b,3c,4,6,与国际上已经报道的大豆疫霉菌株的毒力公式不同,为新的生理小种.该研究可为抗病品种的选育及利用提供科学依据.
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| 关 键 词： | 大豆根腐病 大豆疫霉 生理小种 |
Identification and isolation of soybean root rot in Nanjing |
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| WU Xiao-ling,ZHAO Jin-ming,WANG Yong-lin,SUN Shi,GUO Na,TANG Qing-hua,WANG Yuan-chao,GAI Jun-yi,XING Han.Identification and isolation of soybean root rot in Nanjing[J].Journal of Nanjing Agricultural University,2009,32(2). |
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| Authors: | WU Xiao-ling ZHAO Jin-ming WANG Yong-lin SUN Shi GUO Na TANG Qing-hua WANG Yuan-chao GAI Jun-yi XING Han |
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| Institution: | 1.National Center of Soybean Improvement/State Key Laboratory of Crop Genetics and Germplasm Enhancement/College ofAgriculture;Nanjing Agricultural University;2.Key Laboratory of Monitoring and Managementof Plant Diseases and Insects;Ministry of Agriculture;Nanjing 210095;China |
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| Abstract: | Soybean root rot caused severely losses on Jiangpu Farm,Nanjing Agricultural University during the growing season of 2005 and 2006.Phytophthora sojae was detected by a rapid and specific PCR in the diseased tissues.Four isolates(PNJ1,PNJ2,PNJ3 and PNJ4)were isolated from the diseased tissues of soybean and soil samples,and identified as Phytophthora sojae Kanufman & Gerdemann.By using the method of hypocotyls inoculation on 14 differential hosts,PNJ1,PNJ2,PNJ3 and PNJ4 were identified as new races,and their... |
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| Keywords: | soybean root rot Phytophthora sojae physiological race |
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