| 绿盲蝽和中黑盲蝽解毒代谢酶活性的检测 |
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| 引用本文： | 武淑文,蒲鑫,吴益东.绿盲蝽和中黑盲蝽解毒代谢酶活性的检测[J].南京农业大学学报,2010,33(5). |
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| 作者姓名： | 武淑文 蒲鑫 吴益东 |
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| 作者单位： | 南京农业大学植物保护学院/农业部作物病虫害监测与防控重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 公益性行业科研专项经费项目(200803011-5) |
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| 摘 要： | 害虫解毒代谢增强是对杀虫剂产生抗性的重要机制。为此,本研究建立了Bt棉田重要害虫绿盲蝽和中黑盲蝽成虫多功能氧化酶、酯酶和谷胱甘肽S转移酶3种解毒酶活性的测定方法。研究结果表明:以DCNB(1,2二氯4硝基苯)为底物,不能检测到棉盲蝽谷胱甘肽S转移酶的活性;棉盲蝽多功能氧化酶活性也不宜选用动力学法进行测定。同时2种棉盲蝽成虫的日龄对解毒酶活性影响不明显。因此,对由不同日龄成虫组成的棉盲蝽田间种群可以直接进行解毒酶活性的检测。
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| 关 键 词： | 绿盲蝽 中黑盲蝽 多功能氧化酶 酯酶 谷胱甘肽S转移酶 |
Determination of activities of detoxification enzymes in Lygus lucorum and Adelphocoris suturalis |
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| WU Shu-wen,PU Xin,WU Yi-dong.Determination of activities of detoxification enzymes in Lygus lucorum and Adelphocoris suturalis[J].Journal of Nanjing Agricultural University,2010,33(5). |
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| Authors: | WU Shu-wen PU Xin WU Yi-dong |
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| Institution: | WU Shu-wen,PU Xin,WU Yi-dong* ( College of Plant Protection/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Enhanced detoxification is an important mechanism of insecticide resistance. In the present study,biochemical assay methods for three kinds of detoxification enzymes ( mixed function oxidases,esterases,and glutathione S-transferases) in two cotton mirids ( Lygus lucorum and Adelphocoris suturalis) were set up. Our results showed that the glutathione S-transferases activities were not detected with DCNB ( 1,2-dichloro-4-nitrobenzene) as substrate,and the mixed function oxidases activities were not measured u... |
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| Keywords: | Lygus lucorum Adelphocoris suturalis mixed function oxidases esterases glutathione S-transferases |
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