| 单克隆抗体用于比较禽呼肠孤病毒株的抗原关系 |
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| 引用本文： | 陈士友,陈浦言.单克隆抗体用于比较禽呼肠孤病毒株的抗原关系[J].南京农业大学学报,1993,16(1):96-99. |
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| 作者姓名： | 陈士友 陈浦言 |
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| 作者单位： | 南京农业大学兽医学系 210014
(陈士友，陈溥言)，南京农业大学兽医学系 210014(蔡宝祥) |
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| 摘 要： |
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| 关 键 词： | 单克隆抗体 禽呼肠孤病毒 禽病 |
COMPARISON OF ANTIGEN RELATIONSHIP OF AVIAN REOVIRUS WITH MONOCLONAL ANTIBODIES |
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| Chen Shiyou Chen Puyan Cai Baoxiang.COMPARISON OF ANTIGEN RELATIONSHIP OF AVIAN REOVIRUS WITH MONOCLONAL ANTIBODIES[J].Journal of Nanjing Agricultural University,1993,16(1):96-99. |
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| Authors: | Chen Shiyou Chen Puyan Cai Baoxiang |
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| Abstract: | Eight strains of hybridoma cell lines secreting McAb against avian reovirus(ARVS)were es-tablished with lymphocyte hybridoma technique.The ELISA titres of McAb from mice ascites fluid were10~(-3).to 10~(-5) Five strains of McAb belong to the subclass IgG1,2 to IgG2b and 1 to IgG3,as identifiedwith immunodiffusion test.The antigen relationship among 4 isolates of ARVS in ELISA-blocking test re-vealed significant difference in antigenic structure.This indicated that the McAb might be useful in compari-son of antigen relationship of ARVS and in typing of ARVS serotypes.Of 4 McAb strains reacting with 2 na-tive isolates(strains Guangzhou and NAU-8902),3 were identical,which suggested a closely relationshipamong them.In addition,McAb D19 can be blocked by all ARVS isolates tested and therefore might beused as a common McAb in survey of ARVS. |
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| Keywords: | cell hybridoma technique monoclonal antibodies specificity identification avian reovirus antigen relationship |
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