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| 应用6个STR基因座进行奶牛亲子鉴定 | |
| 引用本文： | 贾名威,杨利国,管峰,陆汉希,金穗华.应用6个STR基因座进行奶牛亲子鉴定[J].南京农业大学学报,2004,27(1):74-77. |
| 作者姓名： | 贾名威 杨利国 管峰 陆汉希 金穗华 |
| 作者单位： | 1. 南京农业大学动物繁育研究所,江苏,南京,210095 2. 南京农业大学动物繁育研究所,江苏,南京,210095;华中农业大学动物科技学院,湖北,武汉,430070 3. 农业部牛冷冻精液质量检验监督测试中心,江苏,南京,210095 |
| 基金项目： | 国家自然科学基金资助项目 ( 3 0 0 70 5 5 5 ) |
| 摘 要： | 运用PCR技术用BM2113、BM1862、BMc701、BM2934、TGLA122、BM720共6个STR(短串联重复序列)基因座对7头奶牛DNA进行扩增，扩增产物分两组混合后经聚丙烯酰胺凝胶电泳和银染检测，扩增片段互不干扰。采用已确定母女关系的2头奶牛血液样本和5个嫌疑父亲的精液样本，对一头母本已知的奶牛在嫌疑父本中找出父本，并计算父权概率为99．993％。结果证明，银染法检测STR基因座扩增产物可用于奶牛亲子鉴定。 |
| 关 键 词： | 奶牛 亲子鉴定 STR基因座 PCR技术 |
| 文章编号： | 1000-2030(2004)01-0074-04 |
| 修稿时间： | 2003年3月4日 |
Parentage identification of dairy cattle by six STR loci |
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| JIA Ming wei ,YANG Li guo ,GUAN Feng ,LU Han xi ,JIN Sui hua.Parentage identification of dairy cattle by six STR loci[J].Journal of Nanjing Agricultural University,2004,27(1):74-77. | |
| Authors: | JIA Ming wei YANG Li guo GUAN Feng LU Han xi JIN Sui hua |
| Institution: | JIA Ming wei 1,YANG Li guo 1,2*,GUAN Feng 1,LU Han xi 3,JIN Sui hua 3 |
| Abstract: | Amplifications of six short tandem repeat(STR)loci including BM 2113, BM 1862, BMc 701, BM 2934, TGLA 122 and BM 720 on DNA of seven dairy cattles were performed by a PCR(polymerase chain reaction)method.The products of amplification were separated in polyacrylamide gel and analyzed with the sliver staining.The paternity probability was calculated by 99 993% on the basis of blood samples from two cows with confirmed paternity and 5 semen samples unknown paternity.The results showed that the PCR with the sliver staining was a validation method for identification parentage in dairy cattle. |
| Keywords: | short tandem repeat loci dairy cattle parentage identification |
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