氯化盐和激素对发芽蚕豆中γ-氨基丁酸富集的影响

以蚕豆(Vicia faba L.)为试材,研究CaCl2、NaCl、赤霉素(GA3)和脱落酸(ABA)对发芽蚕豆谷氨酸脱羧酶(GAD)、二胺氧化酶(DAO)和γ-氨基丁酸(GABA)的影响,以及多胺(PAs)降解对GABA富集的贡献率。结果表明:低氧胁迫下,除ABA外,CaCl2、NaCl和GA3处理均可提高发芽蚕豆GABA含量;培养液组分中NaCl、CaCl2、GA3浓度分别为37.1 mmol.L-1、7.3 mmol.L-1和27.0μmol.L-1时,发芽蚕豆中GABA富集量(6.14±0.12)mg.g-1是对照的1.58倍;PAs降解对GABA富集的贡献率为37.6%～38.9%。

Effects of chloride salts and hormones on γ-aminobutyric acid enrichment in germinated faba beans( Vicia faba L.)

Effects of CaCl2,NaCl,gibberellic acid(GA3)and abscisic acid(ABA)on glutamate decarboxylase(GAD),diamine oxidases(DAO)and γ-aminobutyric acid(GABA)of germinated faba beans(Vicia faba L.),together with the contribution rate of polyamines(PAs)degradation to the enrichment of GABA were studied.The results showed that CaCl2,NaCl and GA3,except ABA,could increase the content of GABA in germinated faba beans under hypoxia stress.It was indicated that the optimal culture components for GABA enrichment were:NaCl at a concentration of 37.1 mmol·L-1,CaCl2 at a concentration of 7.3 mmol·L-1 and GA3 at a concentration of 27.0 μmol·L-1.Under those conditions,the content of GABA was the highest,reaching(6.14±0.12)mg·g-1,which was 1.58 times of the control.And the contribution rate of PAs degradation to enrichment of GABA ranged from 37.6% to 38.9%.