| 水稻启动子OsN1p的克隆与功能分析 |
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| 引用本文： | 吴智丹,王光,郭玲,李林,刘凤权,邵敏.水稻启动子OsN1p的克隆与功能分析[J].南京农业大学学报,2012,35(2):10-14. |
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| 作者姓名： | 吴智丹 王光 郭玲 李林 刘凤权 邵敏 |
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| 作者单位： | 南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室,江苏南京,210095 |
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| 基金项目： | 国家863计划项目(2007AA10Z188);国家转基因生物新品种培育重大专项(2009ZX08001-005B);国家公益性行业(农业)科研专项(201003021) |
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| 摘 要： | 将OsN1基因上游881 bp启动子(OsN1p)序列取代pBI121中gus基因上游的35S启动子,构建植物表达载体pBIN1p,经农杆菌介导转化水稻品种‘日本晴’,获得转基因植株。GUS组织化学染色结果表明,由该启动子驱动的gus基因能在愈伤组织中较低水平地表达;稻瘟病菌接种转基因植株24 h后,GUS活性为未接种前的4.2倍;5 mmol.L-1水杨酸(SA)和0.5 mmol.L-1茉莉酸甲酯(MeJA)分别喷施转基因植株叶面6 h后,GUS活性分别为处理前的5.9和2.4倍。表明,OsN1p启动子具有启动活性,同时明显具有受稻瘟病菌、SA和MeJA诱导表达的特性。
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| 关 键 词： | 诱导型启动子OsN1p 转基因水稻 gus基因 稻瘟病菌 |
Cloning and functional analysis of the promoter OsN1p of rice |
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| WU Zhi-dan
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WANG Guang
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GUO Ling
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LI Lin
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LIU Feng-quan
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SHAO Min.Cloning and functional analysis of the promoter OsN1p of rice[J].Journal of Nanjing Agricultural University,2012,35(2):10-14. |
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| Authors: | WU Zhi-dan WANG Guang GUO Ling LI Lin LIU Feng-quan SHAO Min |
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| Institution: | (College of Plant Protection/ Key Laboratory of Integrated Management of Crop Diseases and Pests,Ministry of Education,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | The 881 bp putative promoter region of OsN1 gene was isolated from the genomic DNA of’Nipponbare’,named as OsN1p.OsN1p substituted for the 35S promoter in vector pBI121 to construct a new plant expression vector pBIN1p.Transgenic plants were obtained through Agrobacterium-mediated transformation.The analysis of GUS activity showed that the gus expressed low level in transgenic callus.GUS activity in transgenic plants was 4.2 times higher than untreated control when they inoculated 24 h with Magnaporthe grisea;6 h after spraying with 5 mmol·L-1 salicylic acid(SA)or 0.5 mmol·L-1 methyl jasmonate(MeJA),GUS activity in leaves of the transgenic plants was 5.9 and 2.4 times respectively higher than untreated control.It suggested that M.grisea,SA and MeJA were inductive factors for OsN1p promoter. |
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| Keywords: | induced promoter OsN1p transgenic rice gus gene Magnaporthe grisea |
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