|
|||||
|
|
| 巨峰葡萄查尔酮合成酶基因4的克隆及表达特性的RT-PCR分析 | |
| 引用本文： | 周军,陶建敏,彭日荷,熊爱生,蔡斌,徐锦涛,金晓芬,张斌,高峰,高建杰,章镇,姚泉洪.巨峰葡萄查尔酮合成酶基因4的克隆及表达特性的RT-PCR分析[J].南京农业大学学报,2010,33(2). |
| 作者姓名： | 周军 陶建敏 彭日荷 熊爱生 蔡斌 徐锦涛 金晓芬 张斌 高峰 高建杰 章镇 姚泉洪 |
| 作者单位： | 1. 南京农业大学园艺学院,江苏,南京,210095;西南林学院咨源学院,云南,昆明,650224 2. 南京农业大学园艺学院,江苏,南京,210095 3. 上海农业科学院生物技术研究所,上海,201106 4. 南京农业大学园艺学院,江苏,南京,210095;上海农业科学院生物技术研究所,上海,201106 |
| 基金项目： | 江苏省科技厅项目(BG2007309);;农业部公益性行业科研专项(nyzx07-027);;江苏省农业三项工程项目(sx(2007)033,sx(2007)zs39);;上海市科技兴农项目(2006-2-1);;上海市青年科技启明星跟踪计划项目(08QH14021);;云南省重点学科西南林学院森林培育学资助项目 |
| 摘 要： | 克隆了葡萄查尔酮合成酶基因4(CHS4),并采用半定量RT-PCR技术,以actin为内参,分析了CHS4在巨峰葡萄果实发育阶段不同组织的表达。对CHS4的序列分析表明:CHS4含有1个内含子,2个外显子;与CHS1、CHS2、CHS3在核苷酸水平的同源性分别为99.3%、92.6%和76.0%。半定量RT-PCR分析结果表明:CHS4在果皮、果肉、种子、叶片和根系中均有表达,在花后30d的果皮中表达强烈,随后迅速降低,到花后70d表达又增强;CHS4在花后30～45d的果肉中表达强烈,随后迅速降低;在花后45d的种子中也表达强烈,随果实发育,其表达逐渐下降。高温处理抑制CHS4在巨峰葡萄幼叶中的表达,但诱导CHS4在幼根的表达。 |
| 关 键 词： | 查尔酮合成酶基因4 巨峰葡萄 克隆 RT-PCR |
Cloning and expression analysis of CHS4 of Kyoho grape by RT-PCR |
|
| ZHOU Jun,TAO Jian-min,PENG Ri-he,XIONG Ai-sheng,CAI Bin,XU Jin-tao,JIN Xiao-fen,ZHANG Bin,GAO Feng,GAO Jian-jie,ZHANG Zhen,YAO Quan-hong.Cloning and expression analysis of CHS4 of Kyoho grape by RT-PCR[J].Journal of Nanjing Agricultural University,2010,33(2). | |
| Authors: | ZHOU Jun TAO Jian-min PENG Ri-he XIONG Ai-sheng CAI Bin XU Jin-tao JIN Xiao-fen ZHANG Bin GAO Feng GAO Jian-jie ZHANG Zhen YAO Quan-hong |
| Institution: | 1.College of Horticulture/a>;Nanjing Agricultural University/a>;Nanjing 210095/a>;China/a>;2.Biotechnology Research Institute/a>;Shanghai Academy of Agricultural Sciences/a>;Shanghai 201106/a>;3.School of Resource Management/a>;Southwest Forestry College/a>;Kunming 650224/a>;China |
| Abstract: | CHS4(chalcone systhase gene)was cloned and sequence analysis showed that CHS4 contained one intron and two extrons.The homology of on the nucleotide level of CHS4 with CHS1,CHS2 and CHS3 was 99.3%,92.6% and 76.0% respectively.Expression analysis of CHS4 of Kyoho grape was carried out by semi-quantity RT-PCR,using grape actin gene as an internal control.The results showed that CHS4 expressed in berry skin,berry flesh and berry seeds,leaves and roots.CHS4 expressed strongly at 30 day after flowering in berry ... |
| Keywords: | CHS4 Kyoho grape clone RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |