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二花脸猪obese基因3714位点多态性与产仔性状的相关性
引用本文:孙梅,陈杰,赵茹茜,韦习会,刘红林.二花脸猪obese基因3714位点多态性与产仔性状的相关性[J].南京农业大学学报,2004,27(1):71-73.
作者姓名:孙梅  陈杰  赵茹茜  韦习会  刘红林
作者单位:1. 南京农业大学动物科技学院
2. 南京农业大学农业部动物生理生化重点开放实验室,江苏,南京,210095
基金项目:江苏省自然基金重点项目 (BK2 0 0 2 2 0 4),南京农业大学青年科技创新基金 (Y2 0 0 3 0 8)
摘    要:利用Bi PASA技术检测了二花脸猪OB基因的多态性 ,并且分析了其与母猪产仔性能的相关性。结果显示 ,OB基因在第 371 4碱基处存在G/T替换 ,经Bi PASA检测后分别定义为等位基因G和T。在二花脸母猪群中 ,等位基因G和T的频率分别为 0 339和 0 6 6 1。方差分析发现 ,G/G基因型个体的头胎产仔数以及平均产仔数略低于G/T型和T/T型个体 ,但相互之间无显著差异 ;在最高产仔数性状上 ,G/G型个体显著低于G/T型和T/T型个体 (P <0 0 5 )。表明G/G型个体的产仔潜力显著偏低

关 键 词:二花脸猪  obese基因  多态性  产仔性状  相关性  肥胖
文章编号:1000-2030(2004)01-0071-03
修稿时间:2003年1月4日

A potential association between the mutation at 3714 site of obese gene and litter size in Erhualian pig
SUN Mei ,CHEN Jie ,ZHAO Ru qian ,WEI Xi hui ,LIU Hong lin.A potential association between the mutation at 3714 site of obese gene and litter size in Erhualian pig[J].Journal of Nanjing Agricultural University,2004,27(1):71-73.
Authors:SUN Mei  CHEN Jie  ZHAO Ru qian  WEI Xi hui  LIU Hong lin
Institution:SUN Mei 1,CHEN Jie 1,ZHAO Ru qian 2,WEI Xi hui 1,LIU Hong lin 1*
Abstract:The polymorphism of obese (OB) gene locus in 121 Chinese Erhualian sows was investigated by means of Bi PASA.A G/T substitution at base 3 714 in OB gene was identified for allel G and T,and the frequencies of these two alleles were 0 339 and 0 661 respectively.Variance analysis presented that there were no significant difference in the average litter size among G/G,G/T and T/T genotypes,however a significant decrease in reproduction performance was found in the sows with G/G genotype when using the highest litter size of each sows as the index.It was suggested that the sows with G/G genotype possess to relative low prolific potency.
Keywords:Erhualian pig  obese gene  Bidirectional PCR amplification of specific alleles (Bi  PASA)  litter size
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