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| 用PCR、斑点杂交及间接免疫荧光试验诊断鸡传染性贫血的初步研究 | |
| 引用本文： | 孙伟,吴中勤,胡青海,李树香,李刚.用PCR、斑点杂交及间接免疫荧光试验诊断鸡传染性贫血的初步研究[J].南京农业大学学报,1999,22(3):69-72. |
| 作者姓名： | 孙伟 吴中勤 胡青海 李树香 李刚 |
| 作者单位： | 1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京,210095 2. 江苏省家禽研究所,扬州,225007 3. 安徽省贵池市兽医站,贵池,247100 |
| 基金项目： | 农业部 “九五”攻关课题 |
| 摘 要： | 利用地高辛标记的０８５ ｋｂ 的鸡传染性贫血病毒 （ Ｃ Ａ Ｖ） 核酸探针， 对江苏某地区疑为 Ｃ Ａ Ｖ 的１５～３０日龄病鸡的肝 Ｄ Ｎ Ａ 样品进行斑点杂交。结果在被检的２０份样品中有６份为阳性，阳性率为３０％ 。对相同样品根据已知引物（特异性扩增出 Ｃ Ａ Ｖ 的０５８ ｋｂ Ｄ Ｎ Ａ） 进行 Ｐ Ｃ Ｒ 扩增， 所得结果与斑点杂交相同。对应的病鸡血清经间接免疫荧光试验 （ Ｉ Ｆ Ａ）， 有７份为 Ｃ Ａ Ｖ 抗体阳性， 与 Ｐ Ｃ Ｒ 扩增结果的符合率为８３％ 。初步结果表明， 江苏某地区存在 Ｃ Ａ Ｖ 感染。 |
| 关 键 词： | 鸡传染性贫血病毒 聚合酶链反应 核酸探针 间接免疫荧光 |
| 修稿时间： | 1998-10-27 |
Preliminary research on the diagnosis of chicken infecti ous anemia by PCR,dot-blot-hybridization assay and IFA |
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| Sun Wei,Wu Zhongqin,Hu Qinghai,Li Shuxiang,Li Gang.Preliminary research on the diagnosis of chicken infecti ous anemia by PCR,dot-blot-hybridization assay and IFA[J].Journal of Nanjing Agricultural University,1999,22(3):69-72. | |
| Authors: | Sun Wei Wu Zhongqin Hu Qinghai Li Shuxiang Li Gang |
| Abstract: | The chicken anemia virus(CAV)was detected by dot blot hybridization assay using the 0 85 kb PCR products labeled with Digoxigenin as probes.The special fragments(0 58 kb)of CAV DNA were amplified by the polymerase chain reaction(PCR).Using the two methods the tissue DNA samples of Jiangsu Province were detected and the same results showed that 6 out of 20 chicken flocks were positive(the positive rate was 30%).For the serums of the same samples,7 out of 20(35%)were positive by the indirect immunofluorescence assay(IFA).The result of IFA compared with the PCR/dot bloting showed 83% consistency.The preliminary investigation showed that the CAV infection is prevalent in the areas of Jiangsu Province. |
| Keywords: | chicken anemia virus polymerase chain reaction indirect immunofluorescence assay dot bloting |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |