谷氨酰胺的抗疲劳生化机制研究

用两批各 48只小鼠分 4组 (n =12 )每天分别灌喂生理盐水和 3 4mmol·kg-1的谷氨酰胺、谷氨酸和天冬酰胺 ,连续 10d。结果显示 ,三者都可提高小鼠血清、肝和骨骼肌中的肌酸磷酸激酶活性 ,降低乳酸脱氢酶活性 ,并显著增加肝、骨骼肌糖原积累。其中有相同碳架结构的谷氨酰胺、谷氨酸能极显著提高小鼠的游泳耐力 ,但与谷氨酰胺有相等酰胺氮的天冬酰胺作用不强。结果提示 ,谷氨酰胺提高机体抗疲劳作用与其碳架结构有关

Effect and biomechanism of glutamine on fatigue resistance

The effect and mechanism of glutamine on improving fatigue resistance was studied in two experiments.48 mice of each experiment were devided into 4 groups,fed saline and 3.4 mmol*kg－1 Gln,Glu and Asn for 10 days,respectively.Mice swiming lethal experiment and the analysis of CPK,LDH and glycogen in mice serum,liver and muscle were carried out.The results showed that in three experimental groups the CPK activities increased in serum,liver and muscle;the LDH activities descended in liver and muscle.In the treatment groups glycogen contents of liver and muscle were most significantly improved.Moreover Gln and Glu were a little more effective.Mice swiming endurance time of the Gln and the Glu group significantly lasted(P＜0.01)and that of Glu group increased compared with the Asn group(P＜0.05).It is suggested that the effect of Gln on fatigue resistance may be related to its structure of carbon chain.