| 马立克氏病病毒（MDV）B抗原基因的克隆及鉴定 |
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| 引用本文： | 吴贤福,蔡宝祥.马立克氏病病毒（MDV）B抗原基因的克隆及鉴定[J].南京农业大学学报,1996,19(3):99-103. |
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| 作者姓名： | 吴贤福 蔡宝祥 |
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| 作者单位： | 南京农业大学畜禽传染病研究室 |
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| 基金项目： | 国家自然科学基金,高等学校博士点专项科研基金资助项目 |
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| 摘 要： | 将ＭＤＶＧＡ株ＢａｍＨＩ基因文库中含Ｉ３片段的质粒ｐＡＣＹＣ１８４和Ｋ３片段的质粒ｐＨＣ７９扩增，用碱裂解法抽提质粒后，利用相应的限制性内切酶切出目的基因片段，连接到合适的载体上，构建了完整的ＭＤＶＢ抗原基因克隆载体。通过内切酶分析，地高辛（ＤＩＧ）－探针原位杂交，ＤＮＡ序列预测，确认克隆的Ｂ抗原基因正确。
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| 关 键 词： | 鸡病 马立克氏病 病毒 抗原基因 克隆载体 |
CLONING AND IDENTIFICATION OF MDV B ANTIGEN GENE |
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| Wu Xianfu,Cai Baoxiang and Chen Puyan.CLONING AND IDENTIFICATION OF MDV B ANTIGEN GENE[J].Journal of Nanjing Agricultural University,1996,19(3):99-103. |
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| Authors: | Wu Xianfu Cai Baoxiang and Chen Puyan |
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| Abstract: | The gene, 3.9 kb in size, encoding MDV B antigen lies between I 3 and K 3 fragments in highly virulent strain GA BamH I liberary. After selection and amplification, the pACYC 184 I 3 and pHC 79 K 3 plasmids were extracted. The required fragments were selected with correspording restriction endonuclease and inserted into suitable cloning vector by two steps. Finally, the cloned B antigen gene was identified by restriction endonuclease analysis, DIG probe hybridization and DNA sequencing. |
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| Keywords: | MDV B antigen gene liberary cloning vector DIG probe DNA sequencing |
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