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| 半夏凝集素基因克隆及其对桃蚜的抗性研究 | |
| 引用本文： | 吴志明,董文琦,党志红,潘文亮,谢晓亮,温春秀,陈劲枫.半夏凝集素基因克隆及其对桃蚜的抗性研究[J].南京农业大学学报,2010,33(2). |
| 作者姓名： | 吴志明 董文琦 党志红 潘文亮 谢晓亮 温春秀 陈劲枫 |
| 作者单位： | 1. 南京农业大学作物遗传与种质创新国家重点实验室/园艺学院,江苏,南京,210095;河北省农林科学院经济作物研究所,河北,石家庄,050051 2. 河北省农林科学院经济作物研究所,河北,石家庄,050051 3. 河北省农林科学院植物保护研究所,河北,保定,071000 4. 南京农业大学作物遗传与种质创新国家重点实验室/园艺学院,江苏,南京,210095 |
| 基金项目： | 河北省农林科学院青年基金项目(A06030101);;河北省自然科学基金项目(C2009001301);;国家转基因重大专项资助项目(2009ZX08002-005B) |
| 摘 要： | 研究了半夏凝集素(Pinellia ternataagglutinin,PTA)基因对桃蚜(Myzus persicae)的抗性。从半夏幼叶中提取总RNA,采用RT-PCR法分离克隆出PTA1基因cDNA片段,该基因在GenBank中登录号为DQ092435。序列分析表明:该cDNA片段全长为810bp,编码269个氨基酸组成的蛋白,具有单子叶植物甘露糖凝集素基因家族的特征,含有3个由4个氨基酸(QDNY)组成的甘露糖专一结合位点,与报道的天南星科凝集素基因氨基酸序列的同源性在72.7%~92.9%。构建了35S启动子控制下的PTA1基因的植物表达载体pBI121PTA1,该载体含有抗卡那霉素的选择标记基因(nptⅡ),利用根癌农杆菌介导法转化烟草获得抗卡那霉素的植株。PCR和半定量RT-PCR分析表明:PTA1基因已经整合到植物基因组中并在转录水平上得到了有效表达。抗蚜虫鉴定表明:转基因植株有效地抑制了蚜虫的群体增长率,平均抑制率达77.02%,表明该基因对同翅目害虫的抗虫基因工程有重要的应用价值。 |
| 关 键 词： | 半夏凝集素 基因克隆 转基因烟草 抗性 桃蚜 |
The gene cloning of Pinellia ternata agglutinin and its resistance to peach aphids (Myzus persicae) |
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| WU Zhi-ming,DONG Wen-qi,DANG Zhi-hong,PAN Wen-liang,XIE Xiao-liang,WEN Chun-xiu,CHEN Jin-feng.The gene cloning of Pinellia ternata agglutinin and its resistance to peach aphids (Myzus persicae)[J].Journal of Nanjing Agricultural University,2010,33(2). | |
| Authors: | WU Zhi-ming DONG Wen-qi DANG Zhi-hong PAN Wen-liang XIE Xiao-liang WEN Chun-xiu CHEN Jin-feng |
| Institution: | 1.State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture/a>;Nanjing Agricultural University/a>;Nanjing 210095/a>;China/a>;2.Institute of Economic Crop/a>;Hebei Academy of Agricultural and Forestry Sciences/a>;Shijiazhuang 050051/a>;3.Institute of Plant Protection/a>;Baoding 071000/a>;China |
| Abstract: | The effects of PTA gene encoding Pinellia ternata agglutinin(PTA)on the Myzus persicae were studied.Using total RNA isolated from Pinellia ternata young leaves,a cDNA containing PTA-1 gene was amplified by RT-PCR and cloned,and the gene sequence was accepted and released by GenBank with accession number DQ092435.Sequence analysis results showed that this gene is of 810 base pairs,and encoding a lectin protein of 269 anmino acids,it was found that the PTA-1 gene had many common characters of mannose-binding ... |
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