桃遗传多样性的SRAP和SSR标记分析

采用相关序列扩增多态性(SRAP)和简单序列重复多态性(SSR)分子标记,对47份桃(Prunus persica)品种的遗传多样性进行了分析.选用带型清晰的19对SRAP引物和5对SSR引物对47份桃品种的基因组DNA进行扩增,共检测到82个多态性位点.平均每对引物组合产生3.4个多态性位点.应用NTSYS-PC (Version 2.1) 软件采用平均距离法(UPGMA)进行聚类分析.结果表明,47份桃品种的相关系数为0.501～0.842,从总体来看,所选取的47个桃品种相关系数相对较低,遗传多样性比较丰富.对聚类结果分析显示,大部分具有亲缘关系的品种及形态学、生物学特征相近的品种聚在一类,说明聚类分析结果与系谱及生物学特征具有一定的相符性.该研究结果对桃种质资源的鉴定,杂交亲本的选择具有一定的参考价值.

Genetic Diversity Analysis of Prunus persica Using SRAP and SSR Markers

Sequence related amplified polymorphism (SRAP) and simple sequence repeats (SSR) markers were employed to analyze the genetic diversity of Prunus persica varieties.Nineteen SRAP and five SSR primer combinations with clear band pattern and polymorphism were selected,which produced 82 polymorphism loci.The average number of polymorphic loci were 3.4 per primer pairs.Dendrograms were constructed by the unweighted pair group method of arithmetic average (UPGMA) using the NTSYS-PC version 2.1.The genetic similarity coefficient of 47 Prunus persica varieties ranged from 0.501 to 0.842.The low similarity coefficient indicated that there was high level of genetic diversity in the 47 Prunus persica varieties.The most of the varieties with relative relationship in their pedigrees and similar biological characteristics were clustered into the same group.These results provide a beneficial reference for genetic breeding of Prunus persica.