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用SSR标记分析豫综5号和金皇后综合种的遗传变异
引用本文:吴连成,侯本军,库丽霞,王铁固,孟庆雷,陈彦惠.用SSR标记分析豫综5号和金皇后综合种的遗传变异[J].华北农学报,2007,22(3):30-34.
作者姓名:吴连成  侯本军  库丽霞  王铁固  孟庆雷  陈彦惠
作者单位:河南农业大学,农学院,河南,郑州,450002
基金项目:河南省高校杰出科研创新人才工程项目 , 国家高技术研究发展计划(863计划)
摘    要:利用40对SSR标记引物分析了豫综5号和金皇后综合种的遗传变异.结果表明:40对引物在两群体中都扩增出115个多态位点;累加40对引物扩增的基因型种类,豫综5号196种,金皇后194种;豫综5号的平均遗传距离为0.383 4,金皇后综合种为0.339 7;豫综5号的平均观察杂合度是0.382 6,平均期望杂合度是0.474 7;金皇后综合种的平均观察杂合度是0.329 2,平均期望杂合度是0.414 3.从以上结果来看,豫综5号的遗传变异较丰富.

关 键 词:玉米  SSR标记  遗传变异
文章编号:1000-7091(2007)03-0030-05
修稿时间:2006-11-06

Analysis on the Genetic Variation of Yuzong 5 and Gold Queen Maize Population by SSR Marker
WU Lian-cheng,HOU Ben-jun,KU Li-xia,WANG Tie-gu,MENG Qing-lei,CHEN Yan-hui.Analysis on the Genetic Variation of Yuzong 5 and Gold Queen Maize Population by SSR Marker[J].Acta Agriculturae Boreali-Sinica,2007,22(3):30-34.
Authors:WU Lian-cheng  HOU Ben-jun  KU Li-xia  WANG Tie-gu  MENG Qing-lei  CHEN Yan-hui
Institution:Agronomy College, Henan Agricultural University, Zhengzhou 450002, China
Abstract:The genetic variation of Yuzong 5 and Gold queen maize population was analyzed by SSR molecular marker.The results showed that: 115 polymorphic loci from Yuzong 5 and Gold queen maize population were amplified by 40 SSR primers;the genotypic number accumulated by 40 primer amplification were 196 for Yuzong 5 and 194 for Gold queen;the mean genetic distances were 0.383 4 for Yuzong 5 and0.339 7 for Gold queen;the mean observed heterozygosity was 0.382 6 for Yuzong 5 and 0.329 2 for Gold queen,while their mean anticipant heterozygosity was 0.474 7 and 0.414 3,respectively.The results suggested that the genetic variation of Yuzong 5 is more abundant than that of Gold queen maize population.
Keywords:Maize  SSR marker  Genetic variation
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