| 卵黄和低密度脂蛋白对冷冻保存绵羊精子胆固醇、磷脂含量的影响 |
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| 引用本文： | 郑云胜,宏伟,倪利平,田东海,芦永跃,邓常胜,李金泉.卵黄和低密度脂蛋白对冷冻保存绵羊精子胆固醇、磷脂含量的影响[J].华北农学报,2008,23(6). |
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| 作者姓名： | 郑云胜 宏伟 倪利平 田东海 芦永跃 邓常胜 李金泉 |
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| 作者单位： | 内蒙古农业大学,内蒙古,呼和浩特,010018 |
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| 基金项目： | 内蒙古自治区自然科学基金项目(200408020407) |
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| 摘 要： | 为了探索绵羊冷冻精液受精率低的原因，在稀释液中用低密度脂蛋白代替卵黄冷冻绵羊精液，研究LDL对冷冻中精子脂类含量的影响。结果表明，以卵黄稀释液冷冻精液，精子胆固醇含量从鲜精的234．54nmol／10^9降低到163．76nmol／10^9精子，胆固醇与磷脂的比值（c／p）从0．54下降到0．36；以9g／100mL LDL代替卵黄，冷冻后胆固醇含量为243．73nmol／10^9精子，c／p值保持在0．50。研究得出在卵黄稀释液中冷冻绵羊精液，精子胆固醇流失，c／p值下降；以LDL代替卵黄，可有效保护精子胆固醇，避免流失，维持了精子c／p值。
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| 关 键 词： | 绵羊 冷冻精液 卵黄 低密度脂蛋白 磷脂 胆固醇 胆固醇和磷脂比例 |
Effects of Egg Yolk and LDL on Changes of Cholesterol and Phospholipids in Sheep Spermatozoa during Freezing |
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| ZHENG Yun-sheng,HONG Wei,NI Li-ping,TIAN Dong-hai,LU Yong-yue,DENG Chang-sheng,LI Jin-quan.Effects of Egg Yolk and LDL on Changes of Cholesterol and Phospholipids in Sheep Spermatozoa during Freezing[J].Acta Agriculturae Boreali-Sinica,2008,23(6). |
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| Authors: | ZHENG Yun-sheng HONG Wei NI Li-ping TIAN Dong-hai LU Yong-yue DENG Chang-sheng LI Jin-quan |
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| Abstract: | For search the reasons of low pregnant rate of sheep frozen semen,the paper studied on the changes of cholesterol and phospholipids in sheep spermatozoa during freezing,which was protected with egg yolk or low density lipoprotein(LDL)in diluents.The results indicated that the contents of cholesterol of sheep spermatozoa decreased from 234.54 nmol/109 sperm of fresh semen to 163.76 nmol/109 sperm of frozen semen and the ratio of cholesterol and phospholipids(c/p)decreased also from 0.54 to 0.36 when egg yolk... |
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| Keywords: | Sheep Semen frozen Egg yolk Low density lipoprotein(LDL) Phospholipids Cholesterol Ratio of cholesterol and phospholipids(c/p) |
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