花椒组织培养与快速繁殖技术研究

以花椒(ZanthoxyLum bungeanumMaxim.)嫩茎段为外植体进行离体培养,获得了再生植株,并建立起快速繁植无性系。分化的最适培养基为MS 1.0 mg/L 6_BA 0.5 mg/L NAA 2.0 mg/L GA,其增殖系数为3.10;继代增殖最适培养基也为MS 1.0 mg/L 6_BA 0.5 mg/L NAA 2.0 mg/L GA,每代每梢平均增殖数量为2~4个不等;生根最适培养基为1/2 MS 0.1 mg/L IBA 0.2 mg/L IAA,10 d后每株可长出2~4条根。并进行了移栽方面的研究,为大规模快速繁殖青椒提供了一条有效的途径。

Culture in vitro of Zanthoxvlum bungesnum and Rapid Propagation of Regenerated Plantlet

Zanthoxvlum bungesnum tissue culture is conducted by using the new stem of Zanthoxvlum bungesnum.The regenerated plantlet is gained and the unisexual system of rapid propagation is built.The best agar medium to differentiation is MS 1.0 mg/L 6-BA 0.5 mg/L NAA 2.0 mg/L GA;The coefficient of propagation is 3.1;The best agar medium to endosperm-derived is also MS 1.0 mg/L 6-BA 0.5 mg/L NAA 2.0 mg/L GA;The averge number of propagated every plant every generation is 2-4,the best agar medium to root induction is 1/2 MS 0.1 mg/L BA 0.2 mg/L IAA 10 days later,every plant can come out 4-10 roots.The study and the reseach on planting provides a efficient means for rapidly propagating green Zanthoxvlum bungesnum on a large scale.