高丹草种质资源SRAP指纹图谱构建及遗传多样性分析

利用SRAP分子标记技术构建了22份高丹草品种的指纹图谱。该指纹图谱可以准确区分供试的所有22个高丹草品种,置信概率达到99.9 999%,为高丹草品种划分提供了基础。利用筛选出的19个多态性较好的SRAP引物组合对22个高丹草品种进行遗传多样性分析鉴定,共扩增出450个位点,其中多态性位点369个;平均每个引物组合产生23.6个位点和19.4个多态性位点,平均多态性水平为82.2%。通过采用UPGMA方法进行聚类分析,遗传相似系数在0.66～0.94之间,以遗传相似系数0.674为阈值,可将供试材料分为2个类群。

Fingerprint Construction and Genetic Diversity Analysis of Sorghum × Sudan Grass by SRAP Markers

The DNA fingerprint of 22 Sorghum Sudan grass varieties was established by using Sequence-Related Amplified Polymorphism markers(SRAP),with 99.9999% probability of confidence.Using 19 SRAP primer combinations which were selected with abundant polymorphic to analyze genetic diversity among 22 Sorghum Sudan grass.A total of 450 bands were scored by the primer,373 out of them were polymorphic,with an average of 23.6 bands and 19.6 polymorphic bands for each primer combination.The percentage of polymorphic loci was 82.9%.According to the genetic similarity coefficient among different strains and Un-weighted Pair-Group Method Arithmetic Average(UPGMA),Genetic similarities among the 22 Sorghum Sudan grass varieties ranged from 0.43 to 0.93 with an average of 0.66.With the similarity coefficient criteria of 0.674 these 22 accessions were clustered into 2 major groups.The cluster analysis showed that cluster I could be classified into 3 sub-clusters.The results verified very high discrimination ability of SRAP markers,moreover,considering its simple,stable,codominant and well-proportioned distribution,conclusion could be made that SRAP might be a potentially useful marker technique and supplement for SSR in Sorghum Sudan grass hybrid identification.The fingerprinting pattern produced by this study could be applied for identification of the studied genotypes.