影响白三叶草高频率植株再生因素的研究

以白三叶草子叶和下胚轴为外植体,进行直接再生系统中不同基本培养基成分和激素浓度的研究发现：白三叶草下胚轴顶端细胞的分化能力最强,分化率在65％以上,子叶次之,下胚轴其他部位几乎不分化。高维生素和微量元素可有效提高子叶的再生频率15％,而对下胚轴顶端细胞的分化影响不大。在6-BA 3mg／L NAA0．08ms／L的浓度下,下胚轴上段的再生频率最高,达80％以上。

Study on the Factors of Influence the Regeneration Frequent of the Trifolium repens L.

The composition of different fundamental substrates and the different concentration of the hormone for the cotyledon of the Trifolium repens and its hypocotyls taken as the explants were studied. The results show: the strongest differentiation ability of the cellulae on the top of the hypocotyls was more than 65% of the differentiation. The same ability of the cotyledon was right behind it. The rest parts of the hypocotyls have rarely the ability. The higher concentrated vitamins and the minor elements could raise about 15% of the regeneration frequency of the cotyledon , but they have no influence on the top cellulae of the hypocotyls. When the concentration was 6-BA 3 mg/L+NAA 0.08 mg/L the regeneration frequency of the top hypocotyls was more than 80%.