| 水分亏缺条件下豌豆肌动蛋白异型体PEAcII基因的表达 |
| |
| 引用本文： | 张少斌,刘曦,赵依诗,汪澈,刘国琴.水分亏缺条件下豌豆肌动蛋白异型体PEAcII基因的表达[J].华北农学报,2010,25(3). |
| |
| 作者姓名： | 张少斌 刘曦 赵依诗 汪澈 刘国琴 |
| |
| 作者单位： | 1. 沈阳农业大学,生物科学技术学院,辽宁,沈阳,110161
2. 中国农业大学,植物生理学与生物化学国家重点实验室,北京,100094 |
| |
| 基金项目： | 植物生理学与生物化学国家重点实验室开放课题，辽宁省教育厅项目，沈阳农业大学中青年导师基金 |
| |
| 摘 要： | 以培养3～4 d的豌豆幼苗为试验材料,以18S rRNA基因为内参基因,豌豆幼肌动蛋白异型体PEAcII基因为检测基因,在PEG6000模拟水分亏缺条件下,采用半定量逆转录聚合酶链式反应(RT-PCR)体系检测肌动蛋白异型体PEAcII基因在豌豆幼苗中的表达情况。在正常供水条件下,PEAcII在处理的第8 h表达量最高,水分胁迫下,PEA-cII基因在处理的0.5 h表达量下降,在处理的1 h时表达量最高,而后下降。无论是正常供水还是水分胁迫条件下,PEAcII基因的表达都是一个动态变化的过程。试验结果表明,肌动蛋白异型体PEAcII表达量的高低可能与豌豆幼苗适应水分亏缺的生理过程有关,这对于研究豌豆幼苗抗旱性机理以及检测豌豆幼苗的抗旱性强弱具有重要意义。
|
| 关 键 词： | 豌豆 幼苗 肌动蛋白异型体 半定量RT-PCR |
Expression of PEAcII in Pea under Water Deficit |
| |
| ZHANG Shao-bin,LIU Xi,ZHAO Yi-shi,WANG Che,LIU Guo-qin.Expression of PEAcII in Pea under Water Deficit[J].Acta Agriculturae Boreali-Sinica,2010,25(3). |
| |
| Authors: | ZHANG Shao-bin LIU Xi ZHAO Yi-shi WANG Che LIU Guo-qin |
| |
| Institution: | ZHANG Shao-bin1,LIU Xi1,ZHAO Yi-shi1,WANG Che1,LIU Guo-qin2(1.Biological Science and Technology College,Shenyang Agricultural University,Shenyang 110161,China)(2.State Key Laboratory of Plant Physiology and Biochemistry,China Agricultural University,Beijing 100094,China) |
| |
| Abstract: | Plant actins play an important role in many living activity.There are three classes of actin isoforms in pea,different actin isoform perhaps display variant actions in adapting the condition of water deficit.To detect the expression of pea actin isoforms in pea tender under water deficit,semi-quantitative PCR was used.The reference gene was 18S rRNA.Pea seedlings were cultured for 3-4 days in growth chamber.In our experiment,PEG6000 was used to produce water deficit.According to figure 4 and 5,the results s... |
| |
| Keywords: | Pea Tender Actin isoform Semi-quantitative RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
