利用SSR鉴定普通小麦-多枝赖草二体异附加系 Line24中外源染色体同源群的归属

用227对小麦微卫星引物进行PCR扩增，76对可在多枝赖草和耐黄矮病的普通小麦-多枝赖草二体异附加系Line24的小麦亲本中国春、丰抗13间检测到多态性。和多枝赖草相同而与Line24其他小麦亲本不同的扩增带。在这76对引物中，发现有4对引物能从Line24中扩增出进一步用Line24和普通小麦杂交得到的7个不同的单体异附加系进行验证，也得到同样的结果，说明这4对微卫星引物扩增出的特异带可以作为Line24中多枝赖草染色体的分子标记。根据这4对引物各自对应的微卫星标记位点在小麦染色体上的位置，说明Line24中附加的一对多枝赖草染色体是第3，5，6和7部分同源群多枝赖草染色体相互易位形成的。

Identifying the Homoeologous Groups of the Alien Chromosomes of Triticum aestivum- Leymus multicaulis Disomic Chromosome Addition Line Line24 Using SSR Markers

Of the 227 wheat microsatellite prime pairs used to amplify products from Leymus multicaulis,76 showed polymorphisms among Leymus multicaulis, the common wheat parents Chinese Spring and Fengkang No.13 of the Triticum aestivum-Leymus multicaulis disomic chromosome addition line Line 24 which was tolerant to Barley Yellow Dwarf Virus (BYDV). These 76 primer pairs can be useful for identifying the homoeologous groups of the alien Leymus multicaulis chromosomes of Line 24. Among them, 4 could amplify same product from Line 24 and Leymus multicaulis, but different from Chinese spring and Fengkang No.13. The same result was obtained when using 7 different monosomic chromosome addition lines whose parents were Line 24 and different common wheat varieties as template. Therefore, these 4 microsatellites could be used as molecular markers for the alien Leymus multicaulis chromosomes of Line24. According to the loci of the products of those 4 microsatellite prime pairs in wheat, it can be concluded that the Leymus multicaulis chromosomes of Line 24 might contain the fragments of 3Xm(Ns), 5Xm(Ns), 6Xm(Ns) and 7Xm(Ns) chromosomes of Leymus multicaulis. They must have exchanged chromosome fragments each other.