| CD147基因在家兔肠道的克隆与结构预测 |
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| 引用本文： | 贾海丽,韩立强,杨国宇,钟凯,郭豫杰,台玉磊,刘涛,李平,王艳玲.CD147基因在家兔肠道的克隆与结构预测[J].华北农学报,2009,24(1). |
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| 作者姓名： | 贾海丽 韩立强 杨国宇 钟凯 郭豫杰 台玉磊 刘涛 李平 王艳玲 |
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| 作者单位： | 河南农业大学,动物生理生化实验室,河南,郑州,450002 |
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| 摘 要： | 基于电子延伸序列,克隆并分析兔CD147基因.采用兔十二指肠黏膜组织提取总RNA,进行RT-PCR反应,测序并进行结构预测.克隆的兔CD147基因包含一个完整的开放阅读框架,全长为810 bp,编码由270个氨基酸残基组成的CD147前体蛋白,推导的氨基酸序列信号肽为1～16个氨基酸.氨基酸序列与牛、人、褐鼠、野猪的同源性分别为62.5%,65.9%,59.5%和66.3%,三级结构预测表明CD147具有2个免疫球蛋白类似区.试验成功克隆了兔CD147基因,注册到GenBank(Accession.EU650668),并预测其三级结构,为进一步研究其生物学功能奠定基础.
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| 关 键 词： | 兔 克隆 序列分析 结构预测 |
Molecular Cloning and Sequence Analysis of Oryctolagus cuniculus CD147 |
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| JIA Hai-li,HAN Li-qiang,YANG Guo-yu,ZHONG Kai,GUO Yu-jie,TAI Yu-lei,LIU Tao,LI Ping,WANG Yan-ling.Molecular Cloning and Sequence Analysis of Oryctolagus cuniculus CD147[J].Acta Agriculturae Boreali-Sinica,2009,24(1). |
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| Authors: | JIA Hai-li HAN Li-qiang YANG Guo-yu ZHONG Kai GUO Yu-jie TAI Yu-lei LIU Tao LI Ping WANG Yan-ling |
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| Institution: | College of Animal Husbandry and Veterinary Medicine;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | A pair of cloning primers were designed based on the in silico sequence information and were used to clone and analysis the Oryctolagus cuniculus CD147.Total RNA was extracted from the duode num mucosal tissue of Oryctolagus cuniculus and mRNA sequence was amplified by RT-PCR.The positive clone was identified and sequenced.The openreading frame of Oryctolagus cuniculus CD147consists of 810 bp and encodes 270 amino acids.The signal peptide of deduced protein sequence includes 116 amino acids.The predicted pe... |
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| Keywords: | CD147 |
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