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| phaG基因转化马铃薯的初步研究 | |
| 引用本文： | 孙环星,张秀海,吴忠义,黄丛林,李文滨.phaG基因转化马铃薯的初步研究[J].华北农学报,2006,21(1):1-4. |
| 作者姓名： | 孙环星 张秀海 吴忠义 黄丛林 李文滨 |
| 作者单位： | 1. 北京市农林科学院北京农业生物技术研究中心,北京,100089;东北农业大学大豆研究所,黑龙江,哈尔滨,150030 2. 北京市农林科学院北京农业生物技术研究中心,北京,100089 3. 东北农业大学大豆研究所,黑龙江,哈尔滨,150030 |
| 摘 要： | phaG是生产可降解塑料PHAS的关键合成酶基因,利用来源于Burkholderia caryophylli的phaG基因BcphaG,构建了其植物表达载体pTiBHS-2,通过农杆菌(Agrobacterium tumefaciens)介导转化生产上常用的马铃薯(Solanumtuberosum)优良品种大西洋,共得到9株转基因马铃薯。经PCR检测证明外源基因已经整合到植物的基因组上,West-ern blot检测表明BcphaG基因在转基因植物体内已表达。转基因植株在再生初期,苗生长慢,根的再生也很慢,但在生根后生长情况逐渐恢复正常,说明BcphaG基因对再生转基因植株的早期生长有负面影响。 |
| 关 键 词： | 马铃薯 聚中等链长度羟基脂肪酸酯 BcphaG基因 |
| 文章编号： | 1000-7091（2006）01-0001-04 |
| 收稿时间： | 2005-10-12 |
| 修稿时间： | 2005年10月12 |
Production of Agrobacterium-mediated Transgenic Potato Plants Containing phaG Gene |
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| SUN Huan-xing,ZHANG Xiu-hai,WU Zhong-yi,HUANG Cong-lin,LI Wen-bin.Production of Agrobacterium-mediated Transgenic Potato Plants Containing phaG Gene[J].Acta Agriculturae Boreali-Sinica,2006,21(1):1-4. | |
| Authors: | SUN Huan-xing ZHANG Xiu-hai WU Zhong-yi HUANG Cong-lin LI Wen-bin |
| Institution: | 1. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China; 2. Soybean Institute, Northeast Agricultural University, Harbin 150030, China |
| Abstract: | phaG is a key enzyme in PHAs biosynthesis process. In order to produce medium-chain-length polyhydroxyalkanoates(mcl-PHAs)in transgenic plants,plant expression vector pTiBHS-2 containing phaG cloned from Burkholderia caryophylli(BcphaG) was constructed.A major commercial cultivar of potato,Atlantic,was transformed via Agrobacterium tumefaciens mediated procedure.Nine transgenic plants are confirmed positive by PCR analysis.Western blot analysis demonstrated that BcphaG gene is expressed.The effect of over-expressing BcphaG gene on plant growth and development were also analyzed.At the early stage,compared to control plant,the growth of over-expressing lines is inhibited,root regeneration is delayed.However,these transgenic plants recover gradually after roots regenerated.It suggested that plant growth and development is partially affected by over-expressing BcphaG gene. |
| Keywords: | Potato Medium-chain-length polyhydroxyalkanoates phaG gene |
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