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| 与黄瓜抗枯萎病基因连锁的RAPD标记 | |
| 引用本文： | 张海霞,张海英,于广建,张峰,毛爱军,王永健,许勇.与黄瓜抗枯萎病基因连锁的RAPD标记[J].华北农学报,2006,21(2):121-123. |
| 作者姓名： | 张海霞 张海英 于广建 张峰 毛爱军 王永健 许勇 |
| 作者单位： | 1. 东北农业大学,园艺系,黑龙江,哈尔滨,150030;北京蔬菜研究中心,北京,100089 2. 北京蔬菜研究中心,北京,100089 3. 东北农业大学,园艺系,黑龙江,哈尔滨,150030 |
| 基金项目： | 北京市自然科学基金(5052001，5062008) 国家自然科学基金资助(30471186) |
| 摘 要： | 以黄瓜抗枯萎病亲本WIS2757和感枯萎病亲本津研2号及其F2分离群体为试材,采用分离群体分组分析法(BSA)进行了与黄瓜抗枯萎病基因连锁的分子标记研究。运用RAPD技术,利用780条RAPD引物对抗、感亲本进行筛选,其中有113条引物在两亲本之间表现多态性,但仅有引物S49在两组间多态性标记与亲本的多态性标记相同。经F2单株分析,引物S49扩增出的特异DNA片段与WIS2757抗黄瓜枯萎病基因连锁,遗传距离为14 cM。DNA标记条带大约为300 bp,定名为S49-300。 |
| 关 键 词： | 黄瓜 枯萎病 抗性基因 RAPD |
| 文章编号： | 1000-7091（2006）02-0121-03 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-11 |
Identification RAPD Markers Linked to Fusarium Wilt Resistance Gene in Cucumber |
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| ZHANG Hai-xia,ZHANG Hai-ying,YU Guang-jian,ZHANG Feng,MAO Ai-jun,WANG Yong-jian,XU Yong.Identification RAPD Markers Linked to Fusarium Wilt Resistance Gene in Cucumber[J].Acta Agriculturae Boreali-Sinica,2006,21(2):121-123. | |
| Authors: | ZHANG Hai-xia ZHANG Hai-ying YU Guang-jian ZHANG Feng MAO Ai-jun WANG Yong-jian XU Yong |
| Institution: | 1. College of Horticulture, Northeast Agriculture University, Harbin 150030, China ; 2. Beijing Vegetable Research Center, Beijing 100089, China |
| Abstract: | Using RAPD primers with BSA technique screened the RAPD markers linked to Fusarium Wilt resistance gene in Cucumber. The results showed that 113 arbitrary primers can amplified different polymorphism bands between two parents, primer S49 can amplify a specific polymorphism band which is linked to Fusarium Wilt resistance in Cucumber, the genetic distance is 14 cM, and the band is named S49-300. |
| Keywords: | Cucumber Fusarium Wilt Resistance gene RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |