基于CSSSLs的水稻穗长QTL的定位

穗长是影响水稻产量的重要因子之一,是典型的数量性状,遗传基础复杂,且易受环境等因素的影响。染色体单片段代换系(Chromosome single segment substitution lines,CSSSLs)减少了个体间遗传背景的干扰,是鉴定复杂性状QTL的新型遗传材料。本研究以广陆矮4号为受体、日本晴为供体的85个染色体单片段代换系群体为试验材料,通过单因素方差分析和Dunnett’s多重比较测验单片段代换系与受体亲本广陆矮4号之间穗长的差异,对代换片段上穗长QTL进行了鉴定。以P<0.001为阈值,共检测到22个穗长QTLs,分布于除第10染色体以外的11条染色体上,其加性效应值的变化范围为-2.63～3.87,加性效应百分率变化范围为-11.47%～16.88%。这些QTLs的鉴定,为进一步克隆穗长QTL以及水稻穗长的分子改良提供了重要的依据。

Mapping of QTLs for Panicle Length Using CSSSLs in Rice(Oryza sativa L.)

Panicle length is one of the important factors determining rice yield,it is a typical quantitative trait that is liable to be influenced by genetic backgrounds and environments.As a novel research material,chromosome single segment substitution lines are useful in QTL identification because of diminishing the interference of genetic backgrounds among plants.In this study,the QTLs for panicle length were identified with 85 rice CSSSLs derived from Guangluai 4 as recipient and Nipponbare as donor by one-way analysis of variance and Dunnett′s test for means comparisons between CSSSLs and the recipient parent Guangluai 4.A total of 22 QTLs for panicle length were identified on 11 chromosomes except chromosome 10 with a significance of P≤0.001.The additive effect of these QTLs ranged from-2.63 to 3.87,and the additive effect percentages QTLs ranged from-11.47% to 16.88%.The results are important for the QTL cloning and molecular breeding of rice panicle length.