辣椒过氧化物酶同工酶研究及模糊聚类分析

以辣椒属(Capsicum)的7个种(包括4个变种、3个类型)计172个品种为试材,于成株期取功能叶片进行制样分析,得出过氧化物酶(POD)同工酶的基本酶谱为12条酶带,并将172份材料中各个种、类型出现的过氧化物酶同工酶表型频率用模糊聚类分析(fuzzy clusber analysis)方法进行动态聚类.结果表明,C.annuum的两个变种关系极近;C.baccatum的两个变种关系也很近; C.chi-nense与C.frutescens关系较近,这两个种及C.praetermissum与C.annuum关系亦较近,而C.annuum与C.baccatum、eximium C.chacoense关系则较远.从而得出,普通栽培辣椒,首先应该利用其野生种C.annuum var.annuum的有用基因,其次是C.frutescens、C.chinense及C.praetermissum,最后才应当考虑利用C.eximium、C.baccatum及C.chacoense的有用基因.据此对辣椒属的起源、分类及上述种质材料在育种上的应用问题进行了讨论.

Peroxidase Isozyme Study and Fuzzy Cluster Analysis of Capsicum

Polyacrylamide gel electrophoresis was employed in the present study . Functional leaves at adult stage of 172 Capsicum accessions were sampled for peroxidase( POD ) analysis. The basic POD zymogram of Capsicum was first presented on their isozyme performances . 12 isoperoxidase bands were determined in its zymogram. Fuzzy cluster analysis was used in exploring the phenotypic POD isozyme bands frequencies of 7 species and 4 subspecits ( 172 accessions ). It is concluded that the relationship between two subspecies of C. annuum and that of the two subspecies of C. baccatum is very close, respectively. C. chinense was close to C. frutescens while these two species are fairly far from C. praetermissum. In addition, these three taxa are much farther away from C. annuum . The other three specie C. baccatum, C. chacoense and C. eximium have a much greater distance to C. annuum . The present result provides an order of possibility to use the genes from its close relatives in pepper breeding programs . The genes of C. annuum var . minimum var . annuum then follow C. frutescens , C. chinense and C. praetermissum. It is difficult for C. annuum var. annuum to use the genes of Capsicum, such as C. chacoense, C. eximium and C. baccatum .