芸苔链格孢产孢基物与光照条件的选择

在小麦、燕麦、玉米、高粱四种谷物培养基上进行芸苔链格孢Alternariabrassicae(Berk.)Sacc]孢子产生的试验表明,小麦粒及玉米粒培养基是很好的产孢基物.在16天的产孢过程中,每克基物可分别得到166.75、156.65万个孢子.获得孢子期分别为达8及14天,共可诱发出4至7次.按产孢量来看,小麦粒培养基最大,但不及在玉米粒培养基产孢期长而稳定.玉米粒培养基是更合适的芸苔链格孢产孢培养基.用紫外线光灯及日光灯对培养9天的小麦粒产孢基物照射1小时以上即可明显地提高其产孢量.紫外光灯下照射2小时,日光灯下照射2～12小时产孢能力最强.用日光灯照射其产孢总量要高于紫外光灯照射.本项研究为进行白菜种质材料抗芸苔链格孢筛选提供了诱发孢子的可靠方法.

Selection of Medium and Illumination for Sporulation of Alternaria brassicae

The experiment on the possibility of wheat, oat, corn, and sorghum for sporulation of Alternaria brassicae showed that the sterilized wheat and corn grains were suitable media for sporulation.The highest quantity of sporulation was obtained with the wheat grain, while a longer and more stable sporulation was obtained with corn grain. Therefore, the corn grain was the optimal medium for sporulation of A. brassicae. Wheat grains cultured for 9 days under the UV light and fluorescent illumination for over one hour obviously increased the quantity of sporulation. The highest rates of sporulation were obtained when the wheat grain was treated under tge UV-light for 2 hours or under the fluorescent light for 2 to 12 hours. The total output of sporulation under the fluorescent light was higher than that under the UV-light. Through this research, a suitable technique was found for the screening of Chinese cabbage germplasms which are resistant to A. brassicae.