| 冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆 |
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| 引用本文： | 陈丽静,葛菱,李浩戈,郭志富,张丽,党晓璇,陶承光,李天来.冷诱导下毛百合甘油-3-磷酸酰基转移酶活性变化及其基因保守区的克隆[J].华北农学报,2011(5):34-39. |
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| 作者姓名： | 陈丽静 葛菱 李浩戈 郭志富 张丽 党晓璇 陶承光 李天来 |
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| 作者单位： | 沈阳农业大学辽宁省生物技术重点实验室设施园艺省部共建教育部重点实验室;辽宁省农业科学院; |
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| 基金项目： | 辽宁省教育厅项目(L2010495); 中国博士后科学基金面上项目资助(20100471471); 国家高技术研究发展计划(“863”计划)项目(2006AA100109) |
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| 摘 要： | 探讨了野生毛百合甘油-3-磷酸酰基转移酶活性在冷胁迫下的变化,用简并引物扩增了甘油-3-磷酸酰基转移酶基因保守区序列的结果表明,该保守区段长744 bp,推测编码247个氨基酸.在GPAT氨基酸序列中存在1个高度保守的区域(WIAPSGGRDRP),在NCBI上经过BLASTp比对分析,发现这段保守区序列为LPLAT基...
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| 关 键 词： | 毛百合 冷诱导 甘油-3-磷酸酰基转移酶(GPAT) 简并引物 保守区 同源性 |
Change in Glycerol-3-Phosphate Acyltransferase Activity of Lilium dauricum Ker Grawl.and Cloning of Its Conserved cDNA Region under Cold Stress |
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| CHEN Li-jing,GE Ling,LI Hao-ge,GUO Zhi-fu,ZHANG Li,DANG Xiao-xuan,TAO Cheng-guang,LI Tian-lai ,Shenyang Agricultural University,Shenyang ,China.Change in Glycerol-3-Phosphate Acyltransferase Activity of Lilium dauricum Ker Grawl.and Cloning of Its Conserved cDNA Region under Cold Stress[J].Acta Agriculturae Boreali-Sinica,2011(5):34-39. |
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| Authors: | CHEN Li-jing GE Ling LI Hao-ge GUO Zhi-fu ZHANG Li DANG Xiao-xuan TAO Cheng-guang LI Tian-lai Shenyang Agricultural University Shenyang China |
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| Institution: | CHEN Li-jing1,GE Ling1,LI Hao-ge1,GUO Zhi-fu1,ZHANG Li1,DANG Xiao-xuan1,TAO Cheng-guang2,LI Tian-lai1 (1.Bioscience and Technology Institute and Key Laboratory of Protected Horticulture(Ministry of Education),Shenyang Agricultural University,Shenyang 110866,China,2.The Liaoning Academy of Agricultural Sciences,Shenyang 110161,China) |
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| Abstract: | Change in Glycerol-3-Phosphate Acyltransferase(GPAT)activity of Lilium dauricum under cold stress was explored and the conserved sequence of GPAT was amplified using degenerate primers.The results demonstrated that the conserved sequence was 744 bp in length,which was deduced coding 247 amino acids.There is a highly conserved domain(WIAPSGGRDRP)in the amino acid sequence of GPAT.Aligning the conserved domain with BLAST of NCBI,we found it was a catalytic domain of LPLAT gene superfamily enzymes.This family ... |
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| Keywords: | Lilium dauricum Cold stress Glycerol-3-Phosphate acyltransferase Degenerate primers Conserved cDNA region Homology |
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