芦笋离体快速繁殖中嫩茎增殖培养对根分化的影响

本试验对芦笋组织培养中影响生根的培养基激素配比、嫩茎增殖培养中激素配比及嫩茎增殖次数等因素进行了研究。结果表明,嫩茎增殖培养基激素配比和嫩茎增殖次数等因素对后续的茎段生根有重要影响。芦笋组培再生完整植株优化培养过程为:芦笋外植体(茎段)在J2(MS NAA 0.2 mg/L BA 0.5 mg/L)培养中诱导出嫩茎后,再在嫩茎增殖培养基J3(MS NAA 0.2 mg/L BA 0.1 mg/L)或J9(MS NAA 0.05 mg/L BA 0.1 mg/L)增殖1次(嫩茎增殖次数对后续生根影响极大),然后取增殖1次后的茎段接种到生根培养基G4(MS IBA 0.5 mg/L BA 0.05 mg/L 2.0 mg/L PP333)中进行生根培养,最高生根率可达90%以上。芦笋组培苗移栽成活率主要受诱导出的根质量的影响。通过几个因素的优化,最终可使芦笋根诱导率达90%~100%,移植成活率达100%。

Effects of Stem-segment Increased Culture on Rooting Differentiation During Quick Reproduction of Asparagus in vitro

The rooting technique on the formation of asparagus tissue culture system were studied in this experiment,including optimization of hormone combination on rooting cultivation,the effects of the new stem subculture,the frequency of transplanting,optimization of hormone combination on subculture of the new stem and the frequency of transplanting have the great effects on rooting regeneration of the following new stem.The best processes of asparagus in vitro are :After induced on J-2(MS NAA 0.2 mg/L BA 0.5 mg/L),the new stem need subculture one time on J-3(MS NAA 0.2 mg/L BA 0.1 mg/L)or J-(9)(MS NAA 0.05 mg/L BA 0.1 mg/L),Subculture time have the great effect on the following rooting.the new stems which were sub-cultured one time were inoculated on MS IBA 0.5 mg/L BA 0.05(mg/L) PP333 2.0 mg/L for rooting differentiation,the rooting rate was obtained 90%.The quantity of roots affects surviving frequency of tissue culture of asparagus.Though optimizing,the root induced rate is obtained 90%-100%,surviving rate 100% after transplanting.