甜高粱与粒用高粱茎秆生长过程中糖及其代谢相关酶活性的比较

以粒用高粱和甜高粱为材料,对甜高粱与粒用高梁不同时期茎秆的可溶性糖含量、蔗糖含量、果糖含量及SS、SPS酶活力进行比较,结果表明:茎秆中的可溶性糖含量、果糖含量在完熟期甜高粱高于粒用高粱,乳熟期茎秆中蔗糖含量甜高粱高于粒用高粱;两种高粱类型的SPS酶活力变化趋势基本相似,从乳熟期到完熟期粒用高粱茎秆中的SPS酶活力明显增高,SS酶活力在粒用高粱茎秆中随生育期呈上升趋势,甜高粱茎秆中SS酶活力到乳熟期达到最大,明显高于粒用高粱.对糖含量与其相关代谢酶活力的相关性分析表明:甜高粱茎秆中利用SPS酶合成蔗糖的效率要高于粒用高粱;两种类型高粱的SS酶活力与其茎秆中的果糖含量存在明显差异,SS的作用模式不同.

Compare with Sweet Sorghum and Grain Sorghum in the Course of Stem Growth Sugar and Its Metabolism Related Enzyme Activity

In this paper, sweet sorghum and grain sorghum were used to analysis soluble sugar content, sugar con-tent , fructose content and SS, SPS activity. The results showed that soluble sugar content and fructose content of stems in the sweet sorghum are higher than in the grain sorghum at ripening period, sucrose content of stems in sweet sorghum is higher than grain sorghum at milky stage. The change of the activities of sucrose phosphate synthase (SPS) in the two types of sorghums follows a similar trend. The activities of SPS of stems significantly increased in the grain sorghum from the milky period to ripening period.The activities of sucrose synthase (SS)of stems in the grain sorghum is rising during whole growth period. The activities of SS of stems reached maximum in sweet sorghum at milky stage, obviously higher than the grain sorghum. Correlation analysis of sugar content and activities of sugar metabolism enzymes revealed that the efficiency of SPS synthesize sugar in sweet sorghum is higher than grain sorghum. The activities of SS and the fructose content in stems are obviously different in the two types of sorghums, the mode of action of SS is different.