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| 南瓜抗CMV基因的RAPD分子标记筛选 | |
| 引用本文： | 赵福宽,周辉,高遐虹,林成.南瓜抗CMV基因的RAPD分子标记筛选[J].华北农学报,2007,22(1):90-94. |
| 作者姓名： | 赵福宽 周辉 高遐虹 林成 |
| 作者单位： | 1. 北京农学院,生物技术系,北京,102206 2. 新疆师范大学,生命与环境科学学院,新疆,乌鲁木齐,830054 3. 新疆农业大学,园艺学院,新疆,乌鲁木齐,830052 |
| 基金项目： | 北京市教委资助项目 , 北京市农委资助项目 |
| 摘 要： | 以抗病自交系K01和感病自交系K02杂交后自交所得的F2群体为材料,采用分离群体分析法筛选与南瓜抗CMV基因连锁的RAPD分子标记。通过520个随机引物和310组双引物的RAPD扩增分析,共找到了2个与南瓜抗CMV亲本K01中的抗病基因相连锁的分子标记S4391400和S19 S345600。这2个标记与K01的抗病基因的重组率分别为7.5%和11.8%,遗传距离分别为7.1和11.7 cM。 |
| 关 键 词： | 南瓜 CMV RAPD 分子标记 |
| 文章编号： | 1000-7091（2007）01-0090-04 |
| 修稿时间： | 2006-10-18 |
Identification of RAPD Molecular Markers Linked to CMV Resistance Gene in Squash |
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| ZHAO Fu-kuan,ZHOU Hui,GAO Xia-hong,LIN Cheng.Identification of RAPD Molecular Markers Linked to CMV Resistance Gene in Squash[J].Acta Agriculturae Boreali-Sinica,2007,22(1):90-94. | |
| Authors: | ZHAO Fu-kuan ZHOU Hui GAO Xia-hong LIN Cheng |
| Institution: | 1. Biotechnology Department of Beijing Agricultural College, Beijing 102206, China; 2. Life and Environment College of Xinjiang Normal University, Urumqi 830054, China; 3. Horticulture College of Xinjiang Agricultural University, Urumqi 830052, China |
| Abstract: | The study was carried out using a F2 population from a cross between resistant inbred line and susceptible inbred line.Bulked segregant analysis(BSA) was applied to identify RAPD markers linked to the resistance to CMV in squash.520 random primers and 310 double primers with arbitrary sequences were chosen for RAPD amplification.It was found that the polymorphism between the R-bulk and S-bulk could be detected by 6% and 5% of the primers as well as two RAPD markers S439(1400) and S19 S345(600) were linked to the resistance gene of K01 with the overcross value of 7.5% and 11.8%,genetic distance of 7.1 and 11.7 cM. |
| Keywords: | Squash CMV RAPD Molecular marker |
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